Potentially obesogenic diets alter metabolic and neurobehavioural parameters in Wistar rats: a comparison between two dietary models
Potentially obesogenic diets alter metabolic and neurobehavioural parameters in Wistar rats: a comparison between two dietary models
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DOI：
10.1016/j.jad.2020.10.034
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发表时间：
2021-01-15
影响因子：
6.6
通讯作者：
Aquino, Jailane de Souza
中科院分区：
文献类型：
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作者：
Figueiredo do Bonfim, Thais Helena;Tavares, Renata Leite;Aquino, Jailane de Souza
背景：近年来，与肥胖大流行相关的临床研究越来越多，动物研究也被认为具有很大的相关性。然而，尽管文献中已经报道了许多饮食在动物模型中诱导肥胖，但这些模型的有效性证据仍然存在差距，不仅要考虑到体细胞参数的变化，还要考虑到与肥胖相关的合并症的触发。在这种情况下，本研究的目的是比较西方饮食和自助饮食作为肥胖诱导方案的有效性，重点评估Wistar大鼠的代谢、体细胞、氧化、组织学和行为参数。方法：大鼠分别饲喂对照（CON）、西式（WTD）和自助（CAF）三种饮食，为期16周。结果：CAF饮食引起焦虑样行为。CAF组体质量（BMI）、Lee和肥胖指数均升高。CAF和WTD饮食降低葡萄糖和胰岛素耐量，引起血脂异常，增加脂质过氧化，降低肝脏、肾脏和大脑的抗氧化能力。WTD和CAF组脂肪组织中IL-6蛋白表达增加，肝脂肪变性和神经元缺血，而间质性肾炎仅在CAF组出现。结论：CAF饮食在诱导肥胖方面最有效，这既体现在身体参数上，也体现在被评估大鼠中与肥胖相关的代谢和神经行为障碍的数量上。
Background: Clinical studies related to the obesity pandemic have intensified in recent years, being the animal studies are also considered of great relevance. However, despite the fact that many diets have been reported in the literature to induce obesity in animal models, there is still a gap regarding evidence of the efficacy of these models, considering not only changes in somatic parameters, but also the triggering of comorbidities associated with obesity. In this scenario, the aim of this study was to compare the effectiveness of western and cafeteria diets as obesity-inducing protocols, focusing on the evaluation of metabolic, somatic, oxidative, histological and behavioural parameters of Wistar rats.Methods: The rats were fed a control (CON), western (WTD) or cafeteria (CAF) diet for 16 weeks.Results: The CAF diet caused anxiogenic-like behaviour. Body mass (BMI), Lee and adiposity indices increased in the CAF group. CAF and WTD diets reduced glucose and insulin tolerance, caused dyslipidemia, increased lipid peroxidation and decrease antioxidant capacity in the liver, kidneys and brain. The WTD and CAF groups shows greater IL-6 protein expression in adipose tissue, developed hepatic steatosis and ischaemic neurons, whereas interstitial nephritis was observed only in the CAF group.Conclusion: The CAF diet was most effective in inducing obesity, as shown both by the somatic parameters and by the greater number of obesity-related metabolic and neurobehavioural disorders in the evaluated rats.