Regulation of actin cytoskeleton via photolithographic micropatterning
Regulation of actin cytoskeleton via photolithographic micropatterning
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通过光刻微图案调节肌动蛋白细胞骨架
DOI：
10.1142/s1793545822440059
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发表时间：
2022-10
影响因子：
2.5
通讯作者：
Jingjun Xu
中科院分区：
文献类型：
--
作者：
Fulin Xing;Haimei Zhang;Mengyu Li;Hao Dong;Xuehe Ma;Shiyu Deng;Fen Hu;Imshik Lee;Leiting Pan;Jingjun Xu
肌动蛋白细胞骨架在各种细胞功能中起着至关重要的作用。细胞外基质（ECM）可以通过重塑细胞内部骨架来调节细胞形态。为了确定ECM的几何形状如何调节肌动蛋白细胞骨架的组织，我们将单个NIH 3T3细胞接种在具有不同形状和大小的微图案化基底上。结果发现，应力纤维可沿着T形图案的非粘附边缘形成，但在V形图案的开口边缘不存在，表明肌动蛋白细胞骨架的组织依赖于力学环境。此外，在50[公式：见正文][公式：见正文]m圆形图案上观察到次级肌动蛋白环，而在30[公式：见正文][公式：见正文]m和40[公式：见正文][公式：见正文]m图案上未出现次级肌动蛋白环，显示肌动蛋白细胞骨架的大小依赖性组织。成骨细胞、MDCK和A549细胞的肌动蛋白骨架呈T型排列，表明肌动蛋白骨架的排列具有细胞类型特异性。总之，我们的研究结果带来了新的见解肌动蛋白细胞骨架的组织微图案的环境。
Actin cytoskeleton plays crucial roles in various cellular functions. Extracellular matrix (ECM) can modulate cell morphology by remodeling the internal cytoskeleton. To define how geometry of ECM regulates the organization of actin cytoskeleton, we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and sizes. It was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern, but were absent from the opening edge of V-shaped pattern, indicating that the organization of actin cytoskeleton was dependent on the mechanical environment. Furthermore, a secondary actin ring was observed on 50[Formula: see text][Formula: see text]m circular pattern while did not appear on 30[Formula: see text][Formula: see text]m and 40[Formula: see text][Formula: see text]m pattern, showing a size-dependent organization of actin cytoskeleton. Finally, osteoblasts, MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern, suggesting a cell-type specificity in arrangement of actin cytoskeleton. Together, our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments.