Effects of hydrostatic pressure on yeasts isolated from deep-sea hydrothermal vents
Effects of hydrostatic pressure on yeasts isolated from deep-sea hydrothermal vents
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DOI：
10.1016/j.resmic.2015.07.005
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发表时间：
2015-11-01
影响因子：
2.6
通讯作者：
Barbier, Georges
中科院分区：
文献类型：
--
作者：
Burgaud, Gaetan;Hue, Nguyen Thi Minh;Barbier, Georges
静水压力对生物圈中的生命分布起着重要作用。关于深海耐压和（超）嗜压细菌和古菌多样性的知识，以及它们应对高静水压力的具体适应性，已有大量文献记载。最近对深海微生物群落组成的调查显示，出现了意想不到的微型真核生物群落，主要以真菌为主。分子方法，如下一代测序已被用于SSU rRNA基因测序，以揭示真菌类群。目前，海洋真菌学家面临的一个困难但迷人的挑战是创建深海海洋真菌培养物，并评估它们应对压力的能力。事实上，虽然没有普遍的遗传标记的压阻，生理分析提供了具体的相关数据，以估计其适应和理解的作用，真菌群落在深渊。本研究以深海酵母菌为模型，研究了真菌对HHP的形态和生理反应。目的是确定深海酵母是否能够耐受不同的HHP，以及它们是否具有代谢活性。在这里，我们报告了一个意想不到的分类学为基础的二分法的压力与piezosensitve子囊菌和piezotolerant担子菌，和不同的形态开关触发的压力为某些菌株。(C)2015巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
Hydrostatic pressure plays a significant role in the distribution of life in the biosphere. Knowledge of deep-sea piezotolerant and (hyper) piezophilic bacteria and archaea diversity has been well documented, along with their specific adaptations to cope with high hydrostatic pressure (HHP). Recent investigations of deep-sea microbial community compositions have shown unexpected micro-eukaryotic communities, mainly dominated by fungi. Molecular methods such as next-generation sequencing have been used for SSU rRNA gene sequencing to reveal fungal taxa. Currently, a difficult but fascinating challenge for marine mycologists is to create deep-sea marine fungus culture collections and assess their ability to cope with pressure. Indeed, although there is no universal genetic marker for piezoresistance, physiological analyses provide concrete relevant data for estimating their adaptations and understanding the role of fungal communities in the abyss. The present study investigated morphological and physiological responses of fungi to HHP using a collection of deep-sea yeasts as a model. The aim was to determine whether deep-sea yeasts were able to tolerate different HHP and if they were metabolically active. Here we report an unexpected taxonomic-based dichotomic response to pressure with piezosensitve ascomycetes and piezotolerant basidiomycetes, and distinct morphological switches triggered by pressure for certain strains. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.