TEMPORAL AND SPATIAL TRANSITIONS IN COLLAGEN TYPES DURING EMBRYONIC CHICK LIMB DEVELOPMENT
TEMPORAL AND SPATIAL TRANSITIONS IN COLLAGEN TYPES DURING EMBRYONIC CHICK LIMB DEVELOPMENT
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DOI：
10.1016/0012-1606(73)90020-1
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发表时间：
1973-01-01
影响因子：
2.7
通讯作者：
TRELSTAD, RL
中科院分区：
文献类型：
--
作者：
LINSENMA.TF;TOOLE, BP;TRELSTAD, RL
为了确定鸡胚胎肢体发育过程中合成的胶原类型是否发生转变，对鸡胚胎肢体发育不同时期(23~24~40)合成的胶原α链组成进行了分析。组织在~3H-脯氨酸和~3H-赖氨酸存在下孵育，用羧甲基纤维素柱层析测定纯化的标记胶原蛋白的α链分布。我们发现，23-24阶段的小腿间充质主要(如果不是全部)产生(α1)2α2型胶原(链型尚未确定)。大约在第25-26阶段，肢体核心开始合成可检测到的数量的(α1)3胶原，我们推测这是软骨型胶原，[α1(II)]3，而肢体外部主要继续产生(α1)2α2。核心中(α1)3胶原的产生逐渐增加，直到第33阶段，它是在胫骨骨干中唯一可检测到的物种。此后不久(到35+-36阶段)(α1)2α2型胶原在胫骨骨干中重新出现，表明骨胶原[α1(I)]2α2的产生。在接下来的几天孵育中，(α1)2α2在骨骨干中的相对比例增加，而(α1)3仍然是在软骨骨骺中合成的主要物种。
To determine whether transitions occur in the types of collagen synthesized during embryonic chick limb development, the α chain composition of the collagens produced by whole limbs and various anatomical regions of limbs was analyzed at different stages (23–24 to 40). The tissues were incubated in the presence of3H-proline and3H-lysine and the α chain distribution of the purified, labeled collagens was determined by chromatography on carboxymethyl cellulose columns. We found that the stage 23–24 leg mesenchyme is producing predominantly, if not solely, an (α1)2α2 type collagen (chain type as yet undetermined). At about stage 25–26 the limb core begins synthesizing detectable amounts of (α1)3collagen, whichwe presumeto be cartilage type collagen, [α1 (II)]3, while the outer portion of the limb largely continues to produce (α1)2α2. The production of (α1)3collagen in the core progressively increases until, by stage 33 it is the only species detectable in the tibial diaphysis. Shortly thereafter (by stage 35+–36) (α1)2α2 type collagen reappears in the tibial diaphysis signifying the production of bone collagen, [α1 (I)]2α2. During the next several days of incubation, the relative proportion of (α1)2α2 increases in the bony diaphysis while (α1)3remains the predominant species synthesized in the cartilaginous epiphysis.