A SBP-Box Gene VpSBP5 from Chinese Wild Vitis Species Responds to Erysiphe necator and Defense Signaling Molecules
A SBP-Box Gene VpSBP5 from Chinese Wild Vitis Species Responds to Erysiphe necator and Defense Signaling Molecules
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中国野生葡萄品种的 SBP-Box 基因 VpSBP5 对白粉病和防御信号分子有反应
DOI：
10.1007/s11105-013-0591-2
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发表时间：
2013-12-01
影响因子：
2.1
通讯作者：
Xu, Hui
中科院分区：
文献类型：
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作者：
Hou, Hongmin;Yan, Qin;Xu, Hui
从中国野生葡萄属伪网状葡萄（Vitis pseudoreticulata）无性系“百合-35-1”中分离到一个新的SQUAMOSA启动子结合蛋白（SBP）-box基因，命名为VpSBP 5。VpSBP 5的cDNA全长为3，811 bp，编码1，030个氨基酸，含有一个高度保守的SBP结构域，该结构域含有两个C2 HCH型锌结合位点和一个核定位信号。推导的VpSBP 5的氨基酸序列与VvSBP 5的氨基酸序列相同，并与AtSPL 1、AtSPL 12、AtSPL 14、VvSBP 7和VvSBP 17归为同一进化枝（第1组）。我们进一步证实VpSBP 5基因确实靶向细胞核，并具有转录激活活性。用E. necator在E.抗病的百合-35-1和感病的湖南-1，在百合-35-1中表现出更快的反应。此外，水杨酸（SA）和茉莉酸甲酯（MeJA）对百合35-1中VpSBP 5的表达有诱导作用。这些结果表明VpSBP 5可能参与了大肠杆菌对大肠杆菌抗性的调控。葡萄藤品种的抗病性可能与SA和MeJA分子信号的出现时间有关。
A novel SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box gene, designated as VpSBP5, was isolated from Chinese wild Vitis pseudoreticulata clone "Baihe-35-1" infected by Erysiphe necator under natural field conditions. The full-length cDNA sequence of VpSBP5 comprised 3,811 bp and encoded a polypeptide of 1,030 amino acids which contained a highly conserved SBP domain bearing two zinc-binding sites of the C2HCH type and a nuclear localization signal. The deduced amino acid sequence of VpSBP5 was identical to that of VvSBP5 and grouped into the same clade (group 1) with AtSPL1, AtSPL12, AtSPL14, VvSBP7, and VvSBP17. We further confirmed that VpSBP5 gene is indeed targeted to the nucleus and possesses transcriptional activation activity. The expression of VpSBP5 as determined by reverse transcription PCR, was induced by E. necator in the E. necator-resistant V. pseudoreticulata clone Baihe-35-1 and the susceptible clone "Hunan-1," and exhibited a quicker response in Baihe-35-1. Moreover, the expression of VpSBP5 was induced by salicylic acid (SA) and methyl jasmonate (MeJA) in Baihe-35-1. Together, our results indicate that VpSBP5 is likely to participate in the regulation of the resistance to E. necator by inducing SA and MeJA molecular signals in grape, and the degree of disease resistance of the grapevine genotypes may correlate with the time of the peak appearing.