Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins
Universal antibodies and their applications to the quantitative determination of virtually all subtypes of the influenza A viral hemagglutinins
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DOI：
10.1016/j.vaccine.2008.09.015
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发表时间：
2008-11-11
期刊：
影响因子：
5.5
通讯作者：
Li, Xuguang
中科院分区：
文献类型：
--
作者：
Chun, Stella;Li, Changgui;Li, Xuguang
该融合肽是所有16种甲型流感亚型和两种B型流感病毒遗传谱系的血凝素中唯一普遍保守的序列。本研究通过生物信息学方法筛选出具有高疏水性和弱免疫原性的病毒融合肽，并对其进行修饰和偶联，以克服其存在的严重技术障碍。针对融合肽产生的抗体表现出针对病毒序列的显著特异性和即使在严格条件下定量分析病毒血凝素的稳健性。如通过抗体结合实验定量揭示的，在中性pH下展开至生理融合状态时，不同血凝素的融合肽以相同程度暴露。据我们所知，这是第一份使用单一通用抗体定量测定几乎所有流感疫苗的报告。皇冠版权所有（C）2008由爱思唯尔有限公司出版。保留所有权利。
The fusion peptide is the only universally conserved sequence in the hamagglutinins of all 16 subtypes of influenza A and two genetic lineages Of influenza B viruses. Here, peptides selected by bioinformatics approach were modified and conjugated to overcome serious technical hurdles such as the high hydrophobicity and weak immunogenicity of the viral fusion peptides. Antibodies generated against fusion peptides demonstrated remarkable specificity against the viral sequences and robustness of quantitatively analyzing the viral hemagglutinins even under stringent conditions. As quantitatively revealed by anti body-binding experiments, the fusion peptides of diverse hemagglutinins are exposed to the same degree upon unfolding at neutral pH to the physiologically fusogenic state. To our knowledge, this is the first report on the quantitative determination of virtually all influenza vaccines using a single universal antibody. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.