Genetic mapping of 20 novel expressed sequence tags from midgestation mouse embryos suggests chromosomal clustering.
Genetic mapping of 20 novel expressed sequence tags from midgestation mouse embryos suggests chromosomal clustering.
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来自妊娠中期小鼠胚胎的 20 个新表达序列标签的遗传图谱表明染色体聚类。
DOI：
10.1006/geno.1996.4564
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Kingsmore,SF
中科院分区：
文献类型：
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作者：
Tchernev,VT;Barbosa,MD;Detter,JC;Patel,TD;Achey,K;Wakeland,EK;Gueorguieva,RV;Yang,MC;Gossler,A;Kingsmore,SF
目前对哺乳动物胚胎发育过程中调控模式形成和分化过程的基因的认识是有限的。在努力分离发育相关基因，20个新的，末端测序的cDNA从第10.5天postcoplant小鼠胚胎库中选择的亚种间回交小鼠的遗传图谱。20个cDNA克隆中有11个定位于3个小鼠常染色体（Chr 5、11和14），如果胚胎中表达的基因在小鼠基因组中的分布是随机的，则不太可能（P< 0.03）。几个克隆的候选人小鼠发育突变凭借遗传共定位和一致性的胚胎表达模式与突变小鼠中的缺陷分布：Estm11是一个候选人的小鼠突变wabbler致死（wl），因为Estm11映射在附近的wlon小鼠Chr 14和表达在这些地区的胚胎脑中表现出轴突变性inwl。末端序列分析，遗传作图，andin situhybridization似乎是一个有效的组合方法识别和表征的基因在哺乳动物胚胎发育过程中具有潜在的调控功能。
Current knowledge of genes that regulate pattern formation and differentiation processes during mammalian embryonic development is limited. In an effort to isolate developmentally relevant genes, 20 novel, end-sequenced cDNAs selected from a Day 10.5 postcoitum mouse embryo library were genetically mapped in intersubspecific backcross mice. Eleven of 20 cDNA clones mapped to three mouse autosomes (Chr 5, 11, and 14), a result that was unlikely (P< 0.03) if the distribution of genes expressed in embryos is random within the mouse genome. Several clones were candidates for mouse developmental mutations by virtue of genetic colocalization and concordance of embryonic expression patterns with the distribution of defects in mutant mice:Estm11was a candidate for the mouse mutation wabbler-lethal (wl), sinceEstm11mapped in the vicinity ofwlon mouse Chr 14 and was expressed in those regions of embryonic brain that exhibit axonal degeneration inwl.End-sequence analysis, genetic mapping, andin situhybridization appeared to be an effective combination of methods for identification and characterization of genes with potential regulatory functions during mammalian embryogenesis.