SEQUENCE-ANALYSIS OF MOUSE TYROSINASE CDNA AND THE EFFECT OF MELANOTROPIN ON ITS GENE-EXPRESSION
SEQUENCE-ANALYSIS OF MOUSE TYROSINASE CDNA AND THE EFFECT OF MELANOTROPIN ON ITS GENE-EXPRESSION
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DOI：
10.1016/s0006-291x(88)81370-6
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发表时间：
1988-06-30
影响因子：
3.1
通讯作者：
KESTLER, D
中科院分区：
文献类型：
--
作者：
KWON, BS;WAKULCHIK, M;KESTLER, D
以人酪氨酸酶cDNA为探针，分离出约占酪氨酸酶编码区75%的小鼠酪氨酸酶cDNA克隆和包含酪氨酸酶5“编码序列的小鼠基因组克隆，并从这些克隆中确定了小鼠酪氨酸酶基因的核苷酸和推导的氨基酸序列。氨基酸序列分析表明，小鼠酪氨酸酶由533个氨基酸组成，分子量为60，536。该蛋白含有6个潜在的N-糖基化位点、两个富含半胱氨酸和两个富含组氨酸的区域（可能作为铜结合位点）、一个潜在的信号和跨膜序列。小鼠和人酪氨酸酶核苷酸和推导的氨基酸序列为81%同源。用促黑素激素和异丁基甲基黄嘌呤刺激Cloudman S-91黑色素瘤细胞后，小鼠酪氨酸酶mRNA水平升高，其转录水平反映了细胞内酪氨酸酶活性和黑色素含量的变化。
Using human tyrosinase cDNA as a probe, a mouse tyrosinase cDNA clone representing approximately 75% of the tyrosinase coding region and a mouse genomic clone which includes the tyrosinase 5'' coding sequences were isolated: nucleotide and deduced amino acid sequence of the mouse tyrosinase gene were determined from these clones. The predicted amino acid sequence revealed that the mouse tyrosinase is composed of 533 amino acids with a molecular weight of 60,536. The deduced protein contains 6 potential N-glycosylation sites, two cysteine- and two histidine-rich regions which may serve as copper-binding sites, a potential signal and trans-membrane sequences. The mouse and human tyrosinase nucleotide and deduced amino acid sequences are .apprx. 81% homologous. The level of mouse tyrosinase mRNA was elevated after stimulation of Cloudman S-91 melanoma cells with melanotropin and isobutylmethylxanthine and the level of transcript reflected that of tyrosinase activity and melanin content in the cells.