Isolation and extraction of target microbes using thermal sol-gel transformation.
Isolation and extraction of target microbes using thermal sol-gel transformation.
复制标题
使用热溶胶-凝胶转化分离和提取目标微生物。
DOI：
10.1039/b212919a
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
T. Katsuragi
中科院分区：
文献类型：
--
作者：
F. Arai;A. Ichikawa;T. Fukudaa;T. Katsuragi
我们开发了一种新的分离方法，通过使用局部粘度控制从大的异质群体中随机筛选目标微生物。将热溶胶-凝胶转化材料与样品液体混合，并基于通过用电流加热微电极和聚焦激光照射靶附近来控制温度变化来可逆地控制从溶胶到凝胶和凝胶到溶胶的状态。所选择的微生物通过凝胶固定在底板上，因为目标周围的粘度通过聚焦激光的局部加热而暂时增加。其他物体很容易被微室中的清洗流冲走。目的微生物的固定、清洗、分离和提取过程在很短的时间内完成。基于这种方法，我们研制了两套分离系统，并给出了靶的固定和分离的基本实验结果。
We developed a novel separation method for random screening of target microorganisms from a large heterogeneous population by using a local viscosity control. A thermal sol-gel transformation material is mixed with the sample liquid and we controlled the state from sol to gel and gel to sol reversibly based on the temperature change controlled by heating of the microelectrode with the electric current and focused laser irradiation near the target. The selected microorganisms are fixed on the bottom plate by gel, since the viscosity around the target is temporally increased by the local heating by the focused laser. The other objects are easily washed away by the cleaning flow in the microchamber. Process of fixation, cleaning, isolation and extraction of the target microbe was possible in very short time. Based on this method, two separation systems are developed and basic experimental results of fixation and isolation of targets are shown.