Expression of SRSF3 is Correlated with Carcinogenesis and Progression of Oral Squamous Cell Carcinoma.
Expression of SRSF3 is Correlated with Carcinogenesis and Progression of Oral Squamous Cell Carcinoma.
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SRSF3的表达与口腔鳞状细胞癌的发生、进展相关
DOI：
10.7150/ijms.14871
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发表时间：
2016
影响因子：
3.6
通讯作者：
Rong J
中科院分区：
文献类型：
--
作者：
Peiqi L;Zhaozhong G;Yaotian Y;Jun J;Jihua G;Rong J
目的：口腔鳞状细胞癌（OSCC）是头颈部最常见的恶性肿瘤，死亡率高。OSCC的发生和发展机制在很大程度上仍不清楚。前mrna选择性剪接失调与OSCC有关。剪接因子SRSF3是一种原癌基因，在多种癌症中过表达。本研究的目的是揭示SRSF3与口腔鳞状细胞癌的发生和发展之间的关系。设计与方法：采用免疫组化方法分析SRSF3在口腔正常组织、非典型增生组织和癌组织中的表达。实时定量RT-PCR检测emt相关基因的表达水平。western blot检测经DMBA处理的原代培养口腔上皮细胞中SRSF3的表达。结果：SRSF3在口腔癌及中重度发育不良组织中过表达。高恶性肿瘤或淋巴转移患者SRSF3表达上调。在体外实验中，敲低SRSF3可抑制Snail和N-cadherin的表达。致癌物DMBA处理的原代培养口腔上皮细胞的SRSF3水平明显高于对照细胞。结论：我们的研究结果提示SRSF3与OSCC的发生和发展有关，可能是OSCC的生物标志物和治疗靶点。
Objective: Oral squamous cell carcinoma (OSCC) is the most common malignancy of head and neck with high mortality rates. The mechanisms of initiation and development of OSCC remain largely unknown. Dysregulated alternative splicing of pre-mRNA has been associated with OSCC. Splicing factor SRSF3 is a proto-oncogene and overexpressed in multiple cancers. The aim of this study was to uncover the relationship between SRSF3 and carcinogenesis and progression of oral squamous cell carcinoma. Design and Methods: The expression of SRSF3 in oral normal, dysplasia, or carcinoma tissues was analyzed by immunohistochemistry. The expression levels of EMT-related genes were quantified by real-time quantitative RT-PCR. The expression of SRSF3 in DMBA treated primary cultured oral epithelial cells were analyzed by western blot. Result: SRSF3 is overexpressed in oral cancer and moderate or severe dysplasia tissues. Patients with high grade cancer or lymphatic metastasis showed up-regulated expression of SRSF3. Knockdown of SRSF3 repressed the expression of Snail and N-cadherin in vitro. Carcinogen DMBA treated primary cultured oral epithelial cells showed significantly increased SRSF3 level than in control cells. Conclusion: Our results suggested that SRSF3 is associated with the initiation and development of OSCC and may be a biomarker and therapeutic target of OSCC.