Identification and determination of succinyladenosine in human cerebrospinal fluid
Identification and determination of succinyladenosine in human cerebrospinal fluid
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DOI：
10.1016/s0378-4347(99)00024-9
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发表时间：
1999-04-16
期刊：
影响因子：
--
通讯作者：
Sebesta, I
中科院分区：
文献类型：
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作者：
Krijt, J;Kmoch, S;Sebesta, I
琥珀酸ladenosine （S-Ado）是嘌呤重新合成的遗传缺陷腺苷酸琥珀酸酶缺乏症的生化标志物。以前有报道称，在没有这种缺陷的儿童的脑脊液中通常检测不到S-Ado。本研究采用固相萃取和薄层色谱法从人脑脊液中分离出一种与S-Ado具有相同光谱和色谱特征的化合物。高效液相色谱-负离子电喷雾质谱分析证实该化合物为S-Ado。采用C-18反相高效液相色谱法建立儿童脑脊液中S-Ado的参考值（1.1 +/- 0.4 μ mol/l，平均+/- S.D, n = 26）。(C) 1999 Elsevier Science B.V.版权所有
Succinyladenosine (S-Ado) is a biochemical marker of adenylosuccinase deficiency - the genetic defect of purine de novo synthesis. S-Ado has been previously reported as normally undetectable in cerebrospinal fluid (CSF) of children not suffering from this defect. In present study, we employed solid-phase extraction and thin-layer chromatography for isolation of a compound with spectral and chromatographic characteristics identical to S-Ado from human CSF. The high performance liquid chromatography-negative-ion electrospray ionization mass spectrometry analysis confirmed that the isolated compound is S-Ado. We established the reference values of S-Ado in CSF of children (1.1 +/- 0.4 mu mol/l; mean +/- S.D; n = 26) by means of reversed-phase HPLC method on a C-18 column with UV detection. (C) 1999 Elsevier Science B.V. All rights reserved.