Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines
Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines
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DOI：
10.1002/elps.200305919
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发表时间：
2004-07-01
期刊：
影响因子：
2.9
通讯作者：
deMello, AJ
中科院分区：
文献类型：
--
作者：
Beard, NP;Edel, JB;deMello, AJ
我们展示了化学反应器与毛细管电泳仪的整体集成，用于快速和灵敏地分析生物胺。异硫氰酸荧光素(FITC)被广泛用于含氨基分析物的分析。然而，缓慢的反应动力学阻碍了这种染料在芯片上的标记应用。也有其他的替代方法，如邻苯二甲醛(OPA)，然而，较差的光物理性质和UV波长(Max)在使用普通激发源时存在困难，导致灵敏度不同。因此，我们首次提出在微流控装置中使用二氯三嗪荧光素(DTAF)作为生物胺的一种优越的原位衍生化试剂。开发的微装置同时采用流体力学和电渗流，促进了聚合物微芯片的创建，以同时执行柱前衍生化和电泳分析。DTAF良好的光物理特性及其快速反应动力学提供了低至1 nm的检测极限和总分析时间(包括芯片上混合和反应)
We demonstrate the monolithic integration of a chemical reactor with a capillary electrophoresis device for the rapid and sensitive analysis of biogenic amines. Fluorescein isothiocyanate (FITC) is widely employed for the analysis of amino-group containing analytes. However, the slow reaction kinetics hinders the use of this dye for on-chip labeling applications. Other alternatives are available such as o-phthaldehyde (OPA), however, the inferior photophysical properties and the UV lambda(max) present difficulties when using common excitation sources leading to a disparity in sensitivity. Consequently, we present for the first time the use of dichlorotriazine fluorescein (DTAF) as a superior in situ derivatizing agent for biogenic amines in microfluidic devices. The developed microdevice employs both hydrodynamic and electroosmotic flow, facilitating the creation of a polymeric microchip to perform both precolumn derivatization and electrophoretic analysis. The favorable photophysical properties of the DTAF and its fast reaction kinetics provide detection limits down to 1 nm and total analysis times (including on-chip mixing and reaction) of