Associations of Human Milk Oligosaccharides and Bioactive Proteins with Infant Morbidity and Inflammation in Malawian Mother-Infant Dyads.
Associations of Human Milk Oligosaccharides and Bioactive Proteins with Infant Morbidity and Inflammation in Malawian Mother-Infant Dyads.
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DOI：
10.1093/cdn/nzab072
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发表时间：
2021-05
影响因子：
4.8
通讯作者：
Dewey KG
中科院分区：
文献类型：
--
作者：
Jorgensen JM;Young R;Ashorn P;Ashorn U;Chaima D;Davis JCC;Goonatilleke E;Kumwenda C;Lebrilla CB;Maleta K;Sadalaki J;Totten SM;Wu LD;Zivkovic AM;Dewey KG
人乳低聚糖(HMOS)和生物活性蛋白可能有益于婴儿健康，但关于这些关系的信息很少。我们的目的是检测HMOS和生物活性蛋白的牛奶含量与婴儿发病率和纵向患病率(任何疾病、发热、腹泻、急性呼吸道感染和食欲不振)以及炎症标志物[C-反应蛋白和α-1-酸性糖蛋白]的关系。这些是一项随机对照试验的二次分析。对产后6个月的母乳样本进行分析，以量化HMOS的绝对丰度、岩藻糖化HMOS、唾液酸化HMOS和51个单独HMOS的相对丰度，以及6种生物活性蛋白(乳蛋白、乳铁蛋白、溶菌酶、抗胰酶、IgA和骨桥蛋白)的浓度。我们检查了这些成分与6至7和6至12个月婴儿发病率的相关性，以及6和18个月时的CRP和AGP，并考虑了母体分泌状态[岩藻糖基转移酶2基因(FUT2)编码的功能酶的存在或不存在]，并调整了协变量和多重假设检验。在秘书中，总HMOS与纵向发热患病率呈正相关(P=0.032)，岩藻糖化HMOS与腹泻发生率呈正相关(P=0.026)，乳铁蛋白与18月时C反应蛋白升高呈正相关(P=0.011)。在非分泌型患者中，乳铁蛋白与发热发生率呈负相关(P=0.007)，骨桥蛋白与食欲不振的纵向发生率呈负相关(P=0.038)，岩藻糖化的HMOS与腹泻发生率(P=0.025)、食欲不振的发生率(P=0.019)、6个月时的AGP和C反应蛋白浓度呈负相关(P=0.001和0.010)；总HMOS与食欲不振(P=0.024)、18月C反应蛋白升高(P=0.026)、乳白蛋白与腹泻发生率(P=0.006)、乳铁蛋白与18月时C反应蛋白升高(P=0.015)呈正相关。某些HMO和生物活性蛋白与婴儿发病率和炎症有关，特别是在非分泌性疾病中。需要进一步的研究来阐明这些关系之间的因果关系。这项试验在Clinicaltrials.gov上注册为NCT01239693。
Human milk oligosaccharides (HMOs) and bioactive proteins likely benefit infant health, but information on these relations is sparse. We aimed to examine associations of milk content of HMOs and bioactive proteins with incidence and longitudinal prevalence of infant morbidity (any illness, fever, diarrhea, acute respiratory infection, and loss of appetite) and markers of inflammation [C-reactive protein (CRP) and α-1-acid glycoprotein (AGP)]. These are secondary analyses of a randomized controlled trial. Breast milk samples at 6 mo postpartum (n = 659) were analyzed to quantify absolute abundance of HMOs, relative abundance of fucosylated HMOs, sialylated HMOs, and 51 individual HMOs, and concentrations of 6 bioactive proteins (lactalbumin, lactoferrin, lysozyme, antitrypsin, IgA, and osteopontin). We examined associations of these constituents with infant morbidity from 6 to 7 and 6 to 12 mo, and CRP and AGP at 6 and 18 mo, considering maternal secretor status [presence or absence of the functional enzyme encoded by the fucosyltransferase 2 gene (FUT2) ] and adjusting for covariates and multiple hypothesis testing. In secretors there were positive associations between total HMOs and longitudinal prevalence of fever (P = 0.032), between fucosylated HMOs and incidence of diarrhea (P = 0.026), and between lactoferrin and elevated CRP at 18 mo (P = 0.011). In nonsecretors, there were inverse associations between lactoferrin and incidence of fever (P = 0.007), between osteopontin and longitudinal prevalence of lost appetite (P = 0.038), and between fucosylated HMOs and incidence of diarrhea (P = 0.025), lost appetite (P = 0.019), and concentrations of AGP and CRP at 6 mo (P = 0.001 and 0.010); and positive associations between total HMOs and incidence of lost appetite (P = 0.024) and elevated CRP at 18 mo (P = 0.026), between lactalbumin and incidence of diarrhea (P = 0.006), and between lactoferrin and elevated CRP at 18 mo (P = 0.015). Certain HMOs and bioactive proteins were associated with infant morbidity and inflammation, particularly in nonsecretors. Further research is needed to elucidate the causality of these relations. This trial was registered at clinicaltrials.gov as NCT01239693.