Efficacy of H5 influenza vaccines produced by reverse genetics in a lethal mouse model
Efficacy of H5 influenza vaccines produced by reverse genetics in a lethal mouse model
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DOI：
10.1086/428951
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发表时间：
2005-04-15
影响因子：
6.4
通讯作者：
Webster, RG
中科院分区：
文献类型：
--
作者：
Lipatov, AS;Webby, RJ;Webster, RG
我们在小鼠身上研究了反向遗传学产生的2H5流感疫苗病毒的有效性。用灭活病毒免疫小鼠，然后接种人H5N1 1997或2003病毒或禽流感H5N1 2001病毒。我们测试的疫苗病毒在单剂疫苗接种后第21天升高了高水平的血凝抑制(1：160~1：1280)和病毒中和抗体(1：900~1：1900)，并减少或阻止了病毒在小鼠肺内的复制；54.5%-100%的免疫小鼠存活，而对照组小鼠全部死亡。尽管抗原性不同，疫苗株与挑战病毒不完全匹配，但仍实现了保护。因此，在小鼠模型中预测到高水平的交叉保护。
We studied the efficacy, in mice, of 2 H5 influenza vaccine viruses produced by reverse genetics. Mice were immunized with inactivated viruses and then inoculated with a human H5N1 1997 or 2003 virus or an avian H5N1 2001 virus. Vaccine viruses that we tested raised high levels of hemagglutination-inhibiting (1:160-1:1280) and virus-neutralizing (1:900-1:1900) antibodies on day 21 after a single dose of vaccine and decreased or prevented virus replication in mouse lungs; 54.5%-100% of immunized mice survived, whereas all control mice died. Protection was achieved despite antigenic differences and incomplete matching of the vaccine strain and the challenge virus. Therefore, high levels of cross-protection are predicted in the mouse model.