An innovative immunochromatography assay for highly sensitive detection of 17β-estradiol based on an indirect probe strategy
An innovative immunochromatography assay for highly sensitive detection of 17β-estradiol based on an indirect probe strategy
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DOI：
10.1016/j.snb.2019.03.078
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发表时间：
2019-06
期刊：
影响因子：
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通讯作者：
Xiaolin Yao;Zonghan Wang;Leina Dou;Bingxin Zhao;Yixin He;Jianlong Wang;Jing Sun;Tao Li;Daohong Zhang
中科院分区：
文献类型：
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作者：
Xiaolin Yao;Zonghan Wang;Leina Dou;Bingxin Zhao;Yixin He;Jianlong Wang;Jing Sun;Tao Li;Daohong Zhang
通过免疫层析测定（伊卡）超灵敏和快速筛选小分子污染物在现场条件下仍然具有挑战性。本文介绍了一种新的基于间接探针的伊卡（ipICA），用于17β-雌二醇（E2）的高灵敏检测。利用羧基修饰的Fe 3 O 4磁性纳米粒子（MNP）标记羊抗鼠抗体（GAMA）。采用游离的抗E2单克隆抗体（mAb）而不是标记的mAb来捕获E2分子。为了比较，还制备了基于MNP的传统和双探针ICA。这种条带传感器表现出更高的灵敏度，最低可见检测限为0.2 ng mL−1，分别比基于MNP的传统条带和双探针条带好5倍和2倍。这种新型传感器的优异性能主要得益于该策略减少了游离mAb的量并放大了信号。该方法已成功应用于牛奶、对虾、鱼和鸡肉样品中雌二醇的检测。该传感器对食品安全监测中的目标分析物的现场检测具有可接受的准确度，对其他小分子污染物也具有潜在的应用价值。
Ultrasensitive and fast screening of small molecule contaminants by immunochromatography assay (ICA) is still challenging in field conditions. Here, a novel indirect probe based ICA (ipICA) for highly sensitive detection of 17β-estradiol (E2) was introduced. The strategy was achieved using carboxyl modified Fe3O4magnetic nanoparticles (MNPs) to label goat anti-mouse antibody (GAMA). The free anti-E2 monoclonal antibody (mAb) rather than labeled mAb was adopted to capture E2 molecules. For comparison, MNPs-based traditional and dual-probe ICAs were also prepared. This strip sensor demonstrated higher sensitivity, with a lowest visible detection limit of 0.2 ng mL−1, which was 5 and 2 times better than that of the MNPs-based traditional strip and dual-probe strip, respectively. The outstanding performance of this novel sensor mainly benefited from the decreased amount of free mAb and the signal amplification of this strategy. Additionally, this method was successfully applied in the detection of E2 in milk, prawn, fish and chicken samples. The proposed sensor exhibited acceptable accuracy for on-site detection of target analyte in food safety monitoring, also has potential applications for the other small molecule contaminants.