DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development
DNA methyltransferase BmDnmt1 and BmDnmt2 in silkworm (Bombyx mori) and the regulation of silkworm embryonic development
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家蚕DNA甲基转移酶BmDnmt1和BmDnmt2及其对家蚕胚胎发育的调控
DOI：
10.1002/arch.21529
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发表时间：
2019-03-01
影响因子：
2.2
通讯作者：
Huang, Shou-Jun
中科院分区：
文献类型：
--
作者：
Li, Bing;Hu, Pei;Huang, Shou-Jun
DNA甲基化是最广泛的表观遗传标记之一，与昆虫发育有关，尤其是影响胚胎发育。然而，DNA甲基化在家蚕胚胎发育和滞育中的调控作用还有待进一步研究。本研究采用逆转录定量聚合酶链反应（RT-PCR）技术检测家蚕DNA甲基转移酶（BmDNMTs）1和2（BmDnmt 1和BmDnmt 2）在家蚕不同组织、不同胚胎发育阶段和不同品系中的表达水平。结果表明，BmDNMTs在胚胎发育过程中表达最高，尤其是在胚胎早期。特别是，BmDNMTs的表达显着上调滞育终止的鸡蛋盐酸处理。组织分布显示，BmDnmt 2在睾丸和卵巢中高表达，BmDnmt 1在睾丸中高表达。本研究有助于了解DNA甲基化与昆虫胚胎发生和配子发生的关系，同时也为进一步分析DNA甲基化在昆虫胚胎发育滞育起始和终止中的作用提供了研究方向。
DNA methylation is one of the most widespread epigenetic marks and has been linked to insect development, especially influencing embryonic development. However, the regulation of DNA methylation in silkworm embryonic development and diapause remain to investigate. In this study, reverse-transcription quantitative polymerase chain reaction was performed to identify the expression level of Bombyx mori DNA methyltransferases (BmDNMTs) 1 and 2 (BmDnmt1 and BmDnmt2) in different tissues, different embryonic developmental stages, and different strains of the silkworm. The results showed that BmDNMTs were the most highly expressed during embryonic development, especially at early embryonic stages. In particular, the expression of BmDNMTs was significantly upregulated in diapause-terminated eggs by HCl treatment. Moreover, tissue distribution showed that BmDnmt2 was highly expressed in testis and ovary, and BmDnmt1 was highly expressed in testis. This study contributes to understanding the correlation of DNA methylation occurs with embryogenesis and gametogenesis in insect, meanwhile, it provides a research orientation to further analyze the role of DNA methylation in diapause initiation and termination in insect embryonic development.