SELECTIVE UPTAKE AND TRANSPORT OF LABEL WITHIN 3 IDENTIFIED NEURONAL SYSTEMS AFTER INJECTION OF H-3-GABA INTO PIGEON OPTIC TECTUM - AUTORADIOGRAPHIC AND GOLGI STUDY
SELECTIVE UPTAKE AND TRANSPORT OF LABEL WITHIN 3 IDENTIFIED NEURONAL SYSTEMS AFTER INJECTION OF H-3-GABA INTO PIGEON OPTIC TECTUM - AUTORADIOGRAPHIC AND GOLGI STUDY
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DOI：
10.1002/cne.901700204
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发表时间：
1976-01-01
影响因子：
2.5
通讯作者：
KUNZLE, H
中科院分区：
文献类型：
--
作者：
HUNT, SP;KUNZLE, H
注射后的3 H-GABA到鸽子[鸽]视顶盖和丘脑，它是可能的相关的某些方面的放射自显影标记模式与观察从高尔基体材料。三个神经元，GABA特异性系统被确定从摄取的氨基酸和从随后的双向细胞内运输的标签。第一个系统来自亚层IIi内的细胞体，其轴突可以在I层内的整个过程中被选择性地标记，并到达前顶盖和腹侧丘脑内的终止区域。放射状上升的树突和轴突侧支的这些神经元内的子层IIf分支，并可以标记后，顶盖或丘脑注射逆行的方式。第二个系统由IIc亚层内的小胞体代表，具有局部和表面定向的树突以及放射状和深定向的轴突，从轴突中产生广泛的轴突侧支系统。这是可能的，直接或间接标记后，这些perikarya切线顶盖注射，优先标记的轴突和终端的这些神经元的更深的区域内的顶盖皮质，并导致逆行轴突运动的标签覆盖的细胞体。在亚层IId内发现了第三个系统，该系统是水平组织的，并且与变性相关，其他放射自显影和高尔基体制剂被认为在本质上主要是树突状的。特定的GABA摄取和随后的运输标签的生化和解剖学意义进行了讨论和模型的顶盖皮质，根据3个建议的抑制系统和它们的关系，一些顶盖传入输入，考虑。
After injection of 3H-GABA into the pigeon [Columba livia] optic tectum and thalamus it was possible to correlate certain aspects of the autoradiographic labeling pattern with observations made from Golgi material. Three neuronal, GABA specific systems were identified both from the uptake of the amino acid and from the subsequent and bidirectional intracellular transport of label. The 1st system derives from cell bodies within sublayer IIi the axons of which could be selectively labeled throughout their course within layer I and to the areas of termination within the pretectum and ventral thalamus. The radially ascending dendrites and axon collaterals of these neurons arborized within sublayer IIf, and could be labeled in a retrograde fashion after tectal or thalamic injections. The 2nd system was represented by small perikarya within sublayer IIc with locally and superficially directed dendrites and with a radially and deep directed axon from which an extensive axon collateral system arose. It was possible to label these perikarya either directly or indirectly after tangential tectal injections which preferentially labeled the axons and terminals of these neurons within the deeper regions of the tectal cortex and resulted in the retrograde axonal movement of label to the overlying cell bodies. A 3rd system was found within sublayer IId, was horizontally organized and from a correlation with degeneration, other autoradiographic and Golgi preparations thought to be mainly dendritic in nature. The biochemical and anatomical implications of specific GABA uptake and subsequent transport of label were discussed and a model of the tectal cortex, based on the 3 proposed inhibitory systems and their relation to a number of tectal afferent inputs, considered.