Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment.
Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment.
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在模拟哺乳动物细胞内环境的条件下培养的耶尔森氏菌的细胞质和膜蛋白。
DOI：
10.1073/pnas.78.2.1224
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发表时间：
1981
影响因子：
11.1
通讯作者：
Brubaker,RR
中科院分区：
文献类型：
--
作者：
Straley,SC;Brubaker,RR
描述了一种将在小培养物中生长的耶尔森氏菌分级分离成内膜和外膜以及可溶性胞质蛋白的方法。该程序被施加到三个公认的物种的生长条件下，模拟哺乳动物细胞内液相对于Ca2+和Mg2+。已知这些条件可引起鼠疫毒力抗原V的产生。通过双向电泳将能够产生该抗原的分离株与毒力抗原阴性衍生物进行比较。V抗原作为与特异性免疫沉淀物的主要组分共迁移的肽定位于可溶性蛋白组分。该肽具有38，000的表观分子量，并且在38，000的表观分子量中均未发现，并且在任一膜级分中均未发现。比较了产毒力抗原和不产毒力抗原的Y。pseudotuberculosis和Y.小肠结肠炎菌的外膜蛋白质模式显示了很大的定性和定量差异，而Y.鼠疫菌只有微小的差别。各种蛋白质组分变化的复杂性证实了文献中的数据，表明在模拟细胞内条件下培养的有毒生物体中发生了广泛的生理变化。
A procedure is described for fractionating Yersinia grown in small cultures into inner and outer membranes and soluble cytoplasmic proteins. The procedure was applied to the three recognized species of the genus grown under conditions simulating mammalian intracellular fluid with respect to Ca2+ and Mg2+. These conditions are known to elicit the production of the plague virulence antigen V. Isolates capable of making this antigen were compared with virulence-antigen-negative derivatives by two-dimensional electrophoresis. The V antigen was localized to the soluble protein fraction as a peptide that comigrates with the major component of a specific immunoprecipitate. This peptide had an apparent molecular weight of 38,000 and was not found in either apparent molecular weight of 38,000 and was not found in either membrane fraction. The comparison of virulence antigen-producers and nonproducers of Y. pseudotuberculosis and Y. enterocolitica revealed large qualitative and quantitative differences in outer membrane protein patterns, whereas the same comparison for Y. pestis showed only minor differences. The complexity of changes in the various protein fractions corroborate data in the literature indicating that extensive physiological changes occur in virulent organisms cultivated under simulated intracellular conditions.