Expression of TGF, matrix metalloproteinases, and tissue inhibitors in Chinese chronic rhinosinusitis
Expression of TGF, matrix metalloproteinases, and tissue inhibitors in Chinese chronic rhinosinusitis
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中国人慢性鼻-鼻窦炎中TGF、基质金属蛋白酶和组织抑制剂的表达
DOI：
10.1016/j.jaci.2010.02.023
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发表时间：
2010-05-01
影响因子：
14.2
通讯作者：
Bachert, Claus
中科院分区：
文献类型：
--
作者：
Li, Xiaoyuan;Meng, Juan;Bachert, Claus
背景资料：到目前为止，还没有关于亚洲慢性鼻炎伴（CRSwNP）或不伴（CRSsNP）鼻息肉患者组织重塑相关介质表达的信息。目的：研究中国CRSwNP和CRSsNP患者TGF-β 1、基质金属蛋白酶（MMPs）、金属蛋白酶组织抑制剂（TIMPs）、胶原和调节性T细胞的表达。36名男性和女性受试者（12名CRSwNP患者，12名CRSsNP患者和12名对照受试者），年龄17至60岁，被招募到研究中。从各组的息肉和窦粘膜、筛粘膜或下鼻甲收集样品，并通过免疫测定法评估TGF-β 1、MMP-2、MMP-7、MMP-9、TIMP-1、TIMP-2、TIMP-3和TIMP-4;通过组织化学评估胶原;通过实时PCR评估叉头盒P3（FOXP3）mRNA。与CRSsNP患者相比，CRSwNP患者的TGF-β 1、TIMP-1、TIMP-4、FOXP3和胶原蛋白浓度显著降低。虽然CRSwNP和CRSsNP患者的MMP-7和MMP-9浓度之间没有显著差异，但与对照患者相比，这些浓度显著增加。MMP-2和TIMP-2的浓度没有显着差异，在任何患者组，而TIMP-3是不可检测的。结论：慢性鼻窦炎鼻息肉的特点是相对缺乏TGF-β表达与CRSsNP。这一发现可能是CRSwNP与CRSsNP相比胶原蛋白、TIMP-1/4和FOXP3表达降低的原因。TGF-β是鼻窦疾病中不同重塑模式的主要开关。（J Allergy Clin Immunol 2010; 125：1061 - 8.）
Background: To date there is no information on the expression of mediators associated with tissue remodeling in Asian patients with chronic rhinitis with (CRSwNP) or without (CRSsNP) nasal polyps.Objectives: To study the expression of TGF-beta 1, matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), collagen, and regulatory T cells in Chinese patients with CRSwNP and CRSsNP.Methods: Thirty-six male and female subjects (12 patients with CRSwNP, 12 patients with CRSsNP, and 12 control subjects), age 17 to 60 years, were recruited into the study. Samples were collected from polyp and sinusoidal mucosal, ethmoidal mucosal, or inferior turbinate in the respective groups and assessed for TGF-beta 1, MMP-2, MMP-7, MMP-9, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 by immunoassay; collagen by histochemistry; and forkhead box P3 (FOXP3) mRNA by real-time PCR.Results: Patients with CRSwNP showed significantly lower concentrations of TGF-beta 1, TIMP-1, TIMP-4, FOXP3, and collagen compared with patients with CRSsNP. Although there were no significant differences between the concentrations of MMP-7 and MMP-9 in patients with CRSwNP and CRSsNP, these were significantly increased compared with control patients. MMP-2 and TIMP-2 concentrations were not significantly different in any patient group, whereas TIMP-3 was not detectable.Conclusion: Chronic rhinosinusitis with nasal polyps is characterized by a relative lack of TGF-beta expression versus CRSsNP. This finding may be causal for decreased collagen, TIMP-1/4, and FOXP3 expression in CRSwNP versus CRSsNP. TGF-beta serves as a main switch for different remodeling patterns in sinus disease. (J Allergy Clin Immunol 2010;125:1061-8.)