RIME proteomics of estrogen and progesterone receptors in breast cancer.
RIME proteomics of estrogen and progesterone receptors in breast cancer.
复制标题
DOI：
10.1016/j.dib.2015.08.019
复制
发表时间：
2015-12
期刊：
影响因子：
1.2
通讯作者：
Mohammed H
中科院分区：
文献类型：
--
作者：
D'Santos C;Taylor C;Carroll JS;Mohammed H
核受体在多种细胞过程的转录调控中发挥重要作用，也与癌症等疾病有关。在乳腺癌中，核受体——雌激素受体（ER）和孕激素受体（PR）是该疾病的经典标志物，并用于分类乳腺癌亚型。利用最近发展的亲和纯化质谱技术（RIME），我们研究了雌激素和黄体酮等配体刺激下乳腺癌细胞株中ER和PR的蛋白相互作用。这些数据被存放在proteomeXchange (PXD002104)，是一篇解释两种核受体之间联系及其在乳腺癌中的潜在后果的出版物的一部分。在这份手稿中，我们描述了所使用的方法，并提供了详细的实验程序，分析方法和原始数据的分析。本文的目的是实现数据的可重复性，并提供在激素环境下进行RIME的技术建议。
Nuclear receptors play an important role in transcriptional regulation of diverse cellular processes and is also relevant in diseases such as cancer. In breast cancer, the nuclear receptors – estrogen receptor (ER) and progesterone receptor (PR) are classical markers of the disease and are used to classify breast cancer subtypes. Using a recently developed affinity purification MS technique (RIME), we investigate the protein interactors of ER and PR in breast cancer cell lines upon stimulation by the ligands – estrogen and progesterone. The data is deposited at proteomeXchange (PXD002104) and is part of a publication that explains the link between the two nuclear receptors and potential consequences of this in breast cancer. In this manuscript, we describe the methodology used and provide details on experimental procedures, analysis methods and analysis of raw data. The purpose of this article is to enable reproducibility of the data and provide technical recommendations on performing RIME in hormonal contexts.