Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization
Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization
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DOI：
10.1016/j.scienta.2020.109581
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发表时间：
2020-10-15
影响因子：
4.3
通讯作者：
Shen, Ren Fang
中科院分区：
文献类型：
--
作者：
Masood, Sajid;Zhao, Xue Qiang;Shen, Ren Fang
一些植物生长促进细菌（PGPB）能够固定大气中的N-2，并可用于减少农业中的氮肥（N）施用。番茄（Solarium lycopersicum L.）施用大量氮肥。但PGPB在番茄氮素营养中的作用研究较少。本研究在温室条件下研究了PGPB改善番茄氮素营养的主要机理。番茄植株在盆中生长，在-N（未施N肥的原生土壤N）或+ N（以尿素形式供应150 mg N kg（-1）干土壤）下，接种或不接种短小芽孢杆菌（PGPB）。施氮促进了番茄的生长、土壤NH 4+浓度和植株对氮的吸收。只有接种B后，氮才能增加根际细菌数量、细菌nifH基因表达和土壤固氮酶活性。pumilus。B。在+ N条件下，接种pumilus可促进番茄生长、氮素吸收、土壤NH 4+浓度、根际细菌数量、土壤细菌基因表达和土壤固氮酶活性。这些结果表明，接种B.在增施氮肥的条件下，矮牵牛促进番茄生长，其原因是矮牵牛增加了根系对B的吸收。短小芽孢杆菌对土壤氮素的固定有辅助作用。
Some plant growth-promoting bacteria (PGPB) are capable of fixing atmospheric N-2 and can be used to reduce nitrogen (N) fertilizer application in agriculture. Large amounts of N fertilizers are applied in tomato (Solarium lycopersicum L.). However, less attention has been given to the role of PGPB in the N nutrition of tomato. The present study was carried out under greenhouse conditions to investigate the principal mechanisms underlying PGPB-improved N nutrition in tomato. Tomato plants were grown in pots under -N (native soil N without N fertilization) or + N (supply 150 mg N kg(-1) dry soil in the form of urea), with or without Bacillus pumilus (PGPB) inoculation. Nitrogen supply improved the growth of tomato, soil NH4+ concentration, and plant N uptake. Nitrogen increased the rhizobacterial population, bacterial nifH gene expression, and soil nitrogenase activity only with the inoculation of B. pumilus. B. pumilus inoculation improved the tomato growth, N uptake, soil NH4+ concentrations, rhizobacterial population levels, soil bacterial gene expression, and soil nitrogenase activity only under + N condition. These results suggest that the inoculation of B. pumilus improves the growth of tomato under the condition of additional fertilizer N supply due to an increase in N uptake by roots from B. pumilus assisted fixed N in soil.