Structural studies on the carbohydrate portion of fetuin.
Structural studies on the carbohydrate portion of fetuin.
复制标题
胎球蛋白碳水化合物部分的结构研究。
DOI：
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发表时间：
1979
影响因子：
4.8
通讯作者：
S. Svensson
中科院分区：
文献类型：
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作者：
B. Nilsson;N. Nordén;S. Svensson
胎儿素的O-糖基连接的碳水化合物链已被碱性硼氢化物处理切断，并与仍然与蛋白质结合的IV-糖基连接的碳水化合物链分离。通过糖和甲基化分析研究了去除乙酰神经氨酸(NeuNAc)残基前后的两种类型的碳水化合物链。通过Smith降解、三氟乙酰解和三氧化二铬氧化后的降解，进一步分析了IV-糖苷键碳水化合物链。作为这些研究的结果，提出了胎儿蛋白碳水化合物链的以下结构：
The O-glycosidically linked carbohydrate chains of fetuin have been cleaved off by alkaline borohydride treatment and separated from the iV-glycosidically linked carbohydrate chains still bound to the protein. Both types of carbohydrate chains have been studied by sugar and methylation analysis before and after removal of Wacetylneuraminic acid (NeuNAc) residues. The iV-glycosidically linked carbohydrate chains have been further analyzed by Smith degradation, trifluoroacetolytic degradation, and degradation after chromium trioxide oxi- dation. As a result of these studies, the following structures for the carbohydrate chains of fetuin are proposed: