Reduced expression of PSCA, a member of the LY-6 family of cell surface antigens, in bladder, esophagus, and stomach tumors
Reduced expression of PSCA, a member of the LY-6 family of cell surface antigens, in bladder, esophagus, and stomach tumors
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DOI：
10.1006/bbrc.2000.3393
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发表时间：
2000-09-07
影响因子：
3.1
通讯作者：
Jakse, G
中科院分区：
文献类型：
--
作者：
Bahrenberg, G;Brauers, A;Jakse, G
前列腺干细胞抗原（PSCA）是在前列腺癌中过度表达的表面蛋白LY-6家族的成员，通过基因表达序列分析（SAGE），我们发现PSCA是分化的尿路上皮肿瘤中最丰富的转录本之一，通过Northern blotting评估，PSCA在正常尿路上皮和非侵袭性尿路上皮肿瘤中高度表达。与之前报道的PSCA在进展性和侵袭性前列腺癌中的过表达相反，我们发现PSCA在未分化的膀胱癌中的表达明显降低。此外，在尿路上皮肿瘤中发现了几种源自PSCA基因的异常剪接产物。此外，PSCA mRNA在正常食管和胃中表达丰富，但在食管和胃肿瘤中检测不到。PSCA的表达似乎依赖于细胞接触，因为当RT112膀胱癌细胞生长到融合时，mRNA水平升高。我们的数据表明PSCA可以作为尿路上皮和胃组织早期癌变的潜在标记物，并且它的表达对上皮细胞是特异性的，(C) 2000学术出版社。
Prostate stem cell antigen (PSCA) is a member of the LY-6 family of surface proteins that is overexpressed in prostate cancer, Using serial analysis of gene expression (SAGE), we identified PSCA as one of the most abundant transcripts in a differentiated urothelial tumor, As assessed by Northern blotting, PSCA is highly expressed in normal urothelium and noninvasive urothelial tumors, In contrast to the previously reported overexpression of PSCA in progressive and invasive forms of prostate cancer, we found a markedly reduced expression in undifferentiated bladder carcinoma. In addition, several aberrant splicing products derived from the PSCA gene were found in urothelial tumors. Furthermore, PSCA mRNA was highly abundant in normal esophagus and stomach, but was undetectable in esophageal or gastric tumors. The PSCA expression appeared to depend on cell contact, since mRNA levels were increased when RT112 bladder carcinoma cells were grown to confluence. Our data suggest that PSCA could serve as a potential marker for the early carcinogenesis in urothelial and gastric tissues and that its expression is specific for epithelial cells, (C) 2000 Academic Press.