Detection of bovine torovirus and other enteric pathogens in feces from diarrhea cases in cattle
Detection of bovine torovirus and other enteric pathogens in feces from diarrhea cases in cattle
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DOI：
10.1177/104063870301500301
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发表时间：
2003-05-01
影响因子：
1.5
通讯作者：
Saif, LJ
中科院分区：
文献类型：
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作者：
Hoet, AE;Nielsen, PR;Saif, LJ
本研究的目的是确定提交给俄亥俄州动物疾病诊断实验室（ADDL）的腹泻病例的牛粪便样本中牛环状病毒（BoTV）的流行率，并评估BoTV与检测到的其他肠道病原体之间是否存在关系。1999年11月至2001年5月，采用抗原捕获酶联免疫吸附试验（ELISA）和逆转录聚合酶链反应（RT-PCR）检测BoTV，对来自53头犊牛（小于或等于6个月）、27头青壮年（大于或等于2岁）、125头成年（大于或等于2岁）和54只年龄未知的动物的259份标本进行了检测。并结合BoTV数据对结果进行分析。9.7%（25/259）的临床样本采用ELISA或RT-PCR检测到BoTV， 56%（14/25）的临床样本来自犊牛（P < 0.001），占犊牛的26.4%（14/53）。在botv阳性犊牛中，71%（10/14）小于3周龄。在11/25的阳性标本中，BoTV是检出的唯一病原体。在小牛样本中单独或与BoTV联合检出的其他肠道生物有轮状病毒、冠状病毒、沙门氏菌、隐孢子虫和贾第鞭毛虫；但没有观察到BoTV与这些生物之间的一致关联。总之，在腹泻牛的粪便样本中检测到BoTV，主要是在3周龄以下的犊牛中。未来对牛传染性腹泻的研究还应包括对该病原体的检测。
The objectives of this study were to determine the prevalence of bovine torovirus (BoTV) in bovine fecal samples from diarrhea cases submitted to the Ohio Animal Disease Diagnostic Laboratory (ADDL) and to assess if a relationship exists between BoTV and the other enteric pathogens detected. From November 1999 to May 2001, 259 specimens from 53 calves (less than or equal to6 months old), 27 young adults (greater than or equal to2 years), 125 adults (greater than or equal to2 years), and 54 animals of unknown age were examined by an antigen-capture enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) assay developed to detect BoTV Testing for other enteric pathogens was performed by ADDL, and the results were analyzed with the BoTV data. The BoTV was detected using ELISA or RT-PCR in 9.7% (25/259) of the clinical samples, 56% (14/25) of which were from calves (P < 0.001) representing 26.4% (14/53) of the calves tested. Of the BoTV-positive calves, 71% (10/14) were less than 3 weeks of age. In 11/25 positive specimens, BoTV was the only pathogen detected among those examined. Other enteric organisms detected alone or in combination with BoTV in calf samples were rotavirus, coronavirus, Salmonella spp., Cryptosporidium spp., and Giardia spp.; but no consistent association between BoTV and these organisms was observed. In summary, BoTV was detected in fecal samples from cattle with diarrhea, principally in young calves less than 3 weeks of age. Future studies of infectious diarrhea in cattle should also include assays for this etiologic agent.