EjNAC3 transcriptionally regulates chilling-induced lignification of loquat fruit via physical interaction with an atypical CAD-like gene.
EjNAC3 transcriptionally regulates chilling-induced lignification of loquat fruit via physical interaction with an atypical CAD-like gene.
复制标题
EjNAC3 通过与非典型 CAD 样基因的物理相互作用转录调节枇杷果实的冷藏诱导木质化
DOI：
10.1093/jxb/erx330
复制
发表时间：
2017-11-02
影响因子：
6.9
通讯作者：
Chen KS
中科院分区：
文献类型：
--
作者：
Ge H;Zhang J;Zhang YJ;Li X;Yin XR;Grierson D;Chen KS
一个新的转录因子EjNAC3通过与CAD类基因直接相互作用参与低温诱导的枇杷木质化，该基因不同于其他“主开关”NAC基因。木质素是许多植物次生细胞壁的重要成分。在枇杷果实中，当果实经受低温贮藏时，肉质组织中的细胞壁发生木质化，这通常用于避免在室温下发生的快速衰老。在这项研究中，分离了两个NAC结构域基因EjNAC3和EjNAC4，并显示在0 °C下被显著诱导，这伴随着果实木质化指数的增加。木质化和表达的EjNAC3和EjNAC4抑制低温条件和热处理。此外，EjNAC3反式激活木质素生物合成相关的EjCAD样启动子，这是使用双荧光素酶测定。进一步的酵母单杂交和电泳迁移率变动分析表明，EjNAC3可以物理结合的EjCAD样基因的启动子。因此，EjNAC3是枇杷低温诱导木质化的直接调节因子，通过调节EjCAD样。
A novel transcription factor, EjNAC3, is involved in chilling-induced lignification in loquat via direct interaction with a CAD-like gene, which differs from other ‘master-switch’ NAC genes. Lignin is an important component of many plant secondary cell walls. In the fruit of loquat (Eriobotrya japonica), lignification of cell walls in the fleshy tissue occurs when fruit are subjected to low-temperature storage, which is commonly used to avoid the rapid senescence that occurs at room temperature. In this study, two NAC domain genes, EjNAC3 and EjNAC4, were isolated and shown to be significantly induced at 0 °C, which was concomitant with an increase in the fruit lignification index. Lignification and expression of both EjNAC3 and EjNAC4 were inhibited by low-temperature conditioning and by heat treatment. In addition, EjNAC3 trans-activated the lignin biosynthesis-related EjCAD-like promoter, which was measured using a dual-luciferase assay. Further analysis with yeast one-hybrid and electrophoretic mobility shift assays indicated that EjNAC3 could physically bind to the promoter of the EjCAD-like gene. Thus, EjNAC3 is a direct regulator of loquat chilling-induced lignification, via regulations of EjCAD-like.
登录
查看更多内容
影响因子：
11.6
作者：
Bennett, Tom;van den Toorn, Albert;Scheres, Ben
通讯作者：
Scheres, Ben
影响因子：
10.5
作者：
Kubo, M;Udagawa, M;Demura, T
通讯作者：
Demura, T
影响因子：
11.6
作者：
Mitsuda, N;Seki, M;Ohme-Takagi, M
通讯作者：
Ohme-Takagi, M
影响因子：
7.2
作者：
Preston, J;Wheeler, J;Parish, RW
通讯作者：
Parish, RW
影响因子：
7
作者：
Xu, Qian;Wang, Wenqiu;Chen, Kunsong
通讯作者：
Chen, Kunsong