Glass bottle sampling solid phase microextraction gas chromatography mass spectrometry for breath analysis of drug metabolites
Glass bottle sampling solid phase microextraction gas chromatography mass spectrometry for breath analysis of drug metabolites
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玻璃瓶进样固相微萃取气相色谱质谱法用于药物代谢物呼气分析
DOI：
10.1016/j.chroma.2017.03.061
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发表时间：
2017
影响因子：
4.1
通讯作者：
Chu Yannan
中科院分区：
文献类型：
--
作者：
Lu Yan;Niu Wenqi;Zou Xue;Shen Chengyin;Xia Lei;Huang Chaoqun;Wang Hongzhi;Jiang Haihe;Chu Yannan
呼吸分析是一种无创的方法，可用于疾病诊断和药代动力学研究。在离线分析的情况下，需要收集呼出的气体，通常使用采样袋作为储存容器。然而，采样袋通常会释放一些额外的化合物，这可能会干扰呼吸测试的结果。在这项研究中，一种新型的呼吸采样玻璃瓶与注射器针头采样端口的固相微萃取（SPME）的开发。这种玻璃瓶几乎不会释放化合物，可用于收集呼出气体，随后通过气相色谱-质谱法（GC-MS）进行分析。采用玻璃瓶采样SPME-GC-MS法对治疗支气管炎和鼻窦炎的复方药物桃金娘酚的呼吸代谢产物进行了研究。在服用桃金娘酚的所有8名志愿者的呼出气中发现了四种化合物，α-蒎烯，2，3-双-1，8-桉叶油素，d-柠檬烯和1，8-桉叶油素。而其他10名未使用桃金娘酚的受试者则检测不到这些化合物。对桃金娘酚的顶空进行SPME-GC-MS分析，检测出α-蒎烯、d-柠檬烯和1，8-桉叶油素3种化合物。通过对呼气和顶空分析结果的比较，表明呼气中的2，3-双-1，8-桉叶素是1，8-桉叶素的代谢产物。这是首次在人体呼吸中发现这种代谢物。研究表明，玻璃瓶采样SPME-GC-MS方法适用于呼出气体分析，包括桃金娘酚等药物的呼吸代谢物研究。
Breath analysis is a non-invasive approach which may be applied to disease diagnosis and pharmacokinetic study. In the case of offline analysis, the exhaled gas needs to be collected and the sampling bag is often used as the storage vessel. However, the sampling bag usually releases some extra compounds, which may interfere with the result of the breath test. In this study, a novel breath sampling glass bottle was developed with a syringe needle sampling port for solid phase microextraction (SPME). Such a glass bottle scarcely liberates compounds and can be used to collect exhaled gas for ensuing analysis by gas chromatography-mass spectrometry (GC–MS). The glass bottle sampling SPME-GC–MS analysis was carried out to investigate the breath metabolites of myrtol, a multicompound drug normally used in the treatment of bronchitis and sinusitis. Four compounds, α-pinene, 2,3-dehydro-1,8-cineole, d-limonene and 1,8-cineole were found in the exhaled breath of all eight volunteers who had taken the myrtol. While for other ten subjects who had not used the myrtol, these compounds were undetectable. In the SPME-GC–MS analysis of the headspace of myrtol, three compounds were detected including α-pinene, d-limonene and 1,8-cineole. Comparing the results of breath and headspace analysis, it indicates that 2,3-dehydro-1,8-cineole in the breath is the metabolite of 1,8-cineole. It is the first time that this metabolite was identified in human breath. The study demonstrates that the glass bottle sampling SPME-GC–MS method is applicable to exhaled gas analysis including breath metabolites investigation of drugs like myrtol.