Differential regulation of testosterone vs. 5alpha-dihydrotestosterone by selective androgen response elements.
Differential regulation of testosterone vs. 5alpha-dihydrotestosterone by selective androgen response elements.
复制标题
选择性雄激素反应元件对睾酮与 5α-二氢睾酮的差异调节。
DOI：
10.1023/a:1007024726889
复制
发表时间：
2000
影响因子：
4.3
通讯作者：
Chang,C
中科院分区：
文献类型：
--
作者：
Hsiao,PW;Thin,TH;Lin,DL;Chang,C
有两种主要的生理雄激素，睾酮(T)和5-二氢睾酮(5-α-dihytesterone，DHT)，它们在哺乳动物中引起不同的反应。这些雄激素通过结合和激活相同的雄激素受体(AR)来调节靶基因的转录。这两种非常接近的雄激素通过相同的AR蛋白来靶向不同的基因组反应的分子机制尚不清楚。通过酵母遗传选择，我们鉴定了两种雄激素反应元件(ARE)，它们可以对T和DHT产生不同的反应。这两种ARE在中国仓鼠卵巢(CHO)细胞中也显示出不同的T-和DHT诱导的AR反式激活的拷贝数，并与经典的小鼠乳腺肿瘤病毒进行了比较。综上所述，我们的结果表明，选择性ARE序列可能在差异T-VS和DHT诱导的AR反式激活中发挥重要作用。
There are two major physiological androgens, testosterone (T), and 5α-dihydrotestosterone (DHT), which induce different responses in mammals. These androgens regulate the target gene transcription via binding to and activating the same androgen receptor (AR). The molecular mechanisms that differ between these two very close androgens through the same AR protein to target the distinct genomic responses remain unknown. Using yeast genetic selection, we identified two kinds of androgen response elements (ARE), which could respond differentially to T vs. DHT. These two AREs also show different T- vs. DHT-induced AR transactivation in mammalian Chinese hamster ovary (CHO) cells in terms of copy number and comparisons with the classic mouse mammary tumor virus ARE. Together, our results suggest that the selective ARE sequence may play an important role in the differential T- vs. DHT-induced AR transactivation.