Overexpression of a TFIIIA‐type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.)
Overexpression of a TFIIIA‐type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.)
复制标题
DOI：
10.1016/j.febslet.2008.02.052
复制
发表时间：
2008-04
期刊：
影响因子：
3.5
通讯作者：
Dongqing Xu;Ji Huang;Shu-qiao Guo;Xia Yang;Y. Bao;Haijuan Tang;Hongsheng Zhang
中科院分区：
文献类型：
--
作者：
Dongqing Xu;Ji Huang;Shu-qiao Guo;Xia Yang;Y. Bao;Haijuan Tang;Hongsheng Zhang
我们以前从水稻中发现了一个盐和干旱胁迫响应的TFIIIA型锌指蛋白基因ZFP252。在这里，我们报告了使用增益型和损失型功能策略对ZFP 252进行功能分析。我们发现，与ZFP 252反义和非转基因植株相比，ZFP 252在水稻中的过表达增加了游离脯氨酸和可溶性糖的含量，提高了胁迫防御基因的表达，提高了水稻对盐和干旱的耐受性。我们的研究结果表明，ZFP252在水稻对盐分和干旱胁迫的响应中发挥着重要作用，并在提高作物对盐分和干旱胁迫的耐受性方面具有实用价值。
We previously identified a salt and drought stress-responsive TFIIIA-type zinc finger protein gene ZFP252 from rice. Here we report the functional analysis of ZFP252 using gain- and loss-of-function strategies. We found that overexpression of ZFP252 in rice increased the amount of free proline and soluble sugars, elevated the expression of stress defense genes and enhanced rice tolerance to salt and drought stresses, as compared with ZFP252 antisense and non-transgenic plants. Our findings suggest that ZFP252 plays an important role in rice response to salt and drought stresses and is useful in engineering crop plants with enhanced tolerance to salt and drought stresses.