THE DYNAMIC-RESPONSE OF VASCULAR ENDOTHELIAL-CELLS TO FLUID SHEAR-STRESS
THE DYNAMIC-RESPONSE OF VASCULAR ENDOTHELIAL-CELLS TO FLUID SHEAR-STRESS
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DOI：
10.1115/1.3138276
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发表时间：
1981-01-01
影响因子：
1.7
通讯作者：
DAVIES, PF
中科院分区：
文献类型：
--
作者：
DEWEY, CF;BUSSOLARI, SR;DAVIES, PF
我们已经开发了一个体外系统，用于研究血管内皮细胞对可控水平的流体剪应力的动态反应。将培养的牛主动脉内皮细胞单层放置在锥板装置中，该装置在复制的样品上产生均匀的流体剪应力。在1~5dynes/cm~2剪切力作用下，亚流合流内皮细胞的增殖速度与静态培养相当，达到相同的饱和密度(≃为1.0~1.5×105个/cm~2)。当受到5-10dynes/cm2的层流剪应力时，融合单层经历了从多边形到椭圆形的随时间变化的细胞形状，并随着流动而变得均匀取向。融合单层中的线状“伤口”的再生似乎受到外力方向的影响。初步研究表明，某些内皮细胞功能，包括液体内吞作用、细胞骨架组装和非血栓形成表面特性，也对切应力敏感。这些观察结果表明，流体机械力可以直接影响内皮细胞的结构和功能。流体剪切力对血管内皮细胞行为的调节可能与正常的血管壁生理以及血管疾病的发病机制有关，如动脉粥样硬化。
We have developed an in-vitro system for studying the dynamic response of vascular endothelial cells to controlled levels of fluid shear stress. Cultured monolayers of bovine aortic endothelial cells are placed in a cone-plate apparatus that produces a uniform fluid shear stress on replicate samples. Subconfluent endothelial cultures continuously exposed to 1–5 dynes/cm2shear proliferate at a rate comparable to that of static cultures and reach the same saturation density (≃ 1.0–1.5 × 105cells/cm2). When exposed to a laminar shear stress of 5–10 dynes/cm2, confluent monolayers undergo a time-dependent change in cell shape from polygonal to ellipsoidal and become uniformly oriented with flow. Regeneration of linear “wounds” in confluent monolayer appears to be influenced by the direction of the applied force. Preliminary studies indicate that certain endothelial cell functions, including fluid endocytosis, cytoskeletal assembly and nonthrombogenic surface properties, also are sensitive to shear stress. These observations suggest that fluid mechanical forces can directly influence endothelial cell structure and function. Modulation of endothelial behavior by fluid shear stresses may be relevant to normal vessel wall physiology, as well as the pathogenesis of vascular diseases, such as atherosclerosis.