BOMBYX-MORI 28S-RIBOSOMAL GENES CONTAIN INSERTION ELEMENTS SIMILAR TO THE TYPE-I AND TYPE-II ELEMENTS OF DROSOPHILA-MELANOGASTER
BOMBYX-MORI 28S-RIBOSOMAL GENES CONTAIN INSERTION ELEMENTS SIMILAR TO THE TYPE-I AND TYPE-II ELEMENTS OF DROSOPHILA-MELANOGASTER
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DOI：
10.1002/j.1460-2075.1985.tb03927.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
ROBINS, B
中科院分区：
文献类型：
--
作者：
EICKBUSH, TH;ROBINS, B
我们已经检查了28S核糖体基因的蚕蛾，家蚕，插入序列的存在。发现两种类型的插入序列，每种都是。5kb长，不共享序列同源性。与未插入的基因的连接区的核苷酸序列的比较表明，一种类型的插入已导致在插入位点的28S编码区的14 bp的重复。该插入和14 bp重复的位置与在黑腹果蝇的I型核糖体插入元件中发现的相同。第二种类型的插入元件位于对应于. apprx的部位。第一种类型的上游75 bp。该插入的位置、在其5“连接处检测到的变异性以及在其3”连接处的短序列同源性区域表明其与D的II型元件相关。黑腹果蝇这是第一个在D.黑腹，和第一个例子的I型样rDNA插入外的高等双翅目。
We have examined the 28S ribosomal genes of the silkmoth, Bombyx mori, for the presence of insertion sequences. Two types of insertion sequences were found, each .apprx. 5 kb in length, which do not share sequence homology. Comparison of the nucleotide sequences of the junction regions with the uninserted gene reveals that one type of insertion has resulted in a 14 bp duplication of the 28S coding region at the insertion site. The location of this insertion and the 14 bp duplication are identical to that found in the Type I ribosomal insertion element of Drosophila melanogaster. The second type of insertion element is located at a site corresponding to .apprx. 75 bp upstream of the first type. The location of this insertion, the variability detected at its 5'' junction, and a short region of sequence homology at its 3'' junction suggest that it is related to the Type II element of D. melanogaster. This is the first example of a Type II-like rDNA insertion outside of sibling species of D. melanogaster, and the first example of a Type I-like rDNA insertion outside of the higher Diptera.