Transcriptomic analyses of chemosensory genes in Trichogramma japonicum (Hymenoptera: Trichogrammatidae)
Transcriptomic analyses of chemosensory genes in Trichogramma japonicum (Hymenoptera: Trichogrammatidae)
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日本赤眼蜂（膜翅目：赤眼蜂科）化学感应基因的转录组分析
DOI：
10.1016/j.cbd.2020.100755
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发表时间：
2021-03-01
影响因子：
3
通讯作者：
Li, Yuan-Xi
中科院分区：
文献类型：
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作者：
Li, Si-Sheng;Yan, Zhi-Chao;Li, Yuan-Xi
昆虫通过嗅觉和味觉感知环境中危险或有吸引力的化学物质（如捕食者，食物或配偶的存在）。这导致了寻找宿主、寻找配偶和其他对昆虫生存至关重要的行为。这些重要的活动是由许多化学感受受体蛋白介导的，包括气味受体（OR），味觉受体（GR）和离子型受体（IR）。日本赤眼蜂Trichogramma japonicum Ashmead是一种重要的水稻卵寄生蜂。为了揭示其嗅觉和味觉系统在生物防治中的作用，我们系统地分析了烟草赤星病菌主要的化学感受受体基因。山茱萸通过对雄性和雌性头部的转录组分析，我们发现差异表达基因（DEG）主要与化学感觉功能相关，并且雌性头部表达的化学感觉相关基因多于雄性头部，这可能与雌性需要有关寻找宿主或感知雄性信息素。此外，我们确定了66个化学感受器基因，其中包括51个OR，7个IR和8个GR，根据Blast best-hit和系统发育分析的结果，与其他种类的昆虫的化学感受器基因的关系最为密切。组织表达谱显示，66个化学感受器中的65个在头部高度表达，表明它们在嗅觉和味觉中的假定作用。此外，性别特异性表达模式表明其在寄主寻找或配偶感应行为中的潜在功能。本研究为进一步了解黑颈长尾雉的嗅觉和味觉系统奠定了基础。提高其在害虫防治中的应用效果。
Insects perceive dangerous or attractive chemicals in the environment (such as the presence of predators, food or mates) through their olfaction and gustation. This leads to host searching, mate finding and other behaviors that are critical for insect survival. These vital activities are mediated by many chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs). Trichogramma japonicum Ashmead (Hymenoptera: Trichogrammatidae) is a commonly used egg parasitoid for controlling lepidopteran pests in rice fields. To reveal the roles of its olfactory and gustatory systems in biological control, we systematically analyzed major chemosensory receptor genes from T. japonicum. Through transcriptomic analyses of male and female heads, we found that the differentially expressed genes (DEGs) were mainly related to chemosensory functions, and there were more chemosensory-related genes expressed in female heads than male heads, which may be related to the need for females to search for their hosts or sense male pheromone. Furthermore, we identified 66 chemosensory receptor genes, including 51 ORs, seven IRs and eight GRs, that were most closely relate to those of other hymenopteran species according to the results of both Blast best-hit and phylogenetic analyses. The tissue expression profile showed that 65 of the 66 chemosensory receptors were highly expressed in the heads, suggesting their putative roles in olfaction and gustation. In addition, the sex specific expression patterns suggested their potential functions in host-seeking or mate sensing behaviors. This study may provide base for further understanding the olfactory and gustatory systems of T. japonicum and increasing its efficiency in pest control in the future.