Correlation between Trop2 and amphiregulin coexpression and overall survival in gastric cancer.
Correlation between Trop2 and amphiregulin coexpression and overall survival in gastric cancer.
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DOI：
10.1002/cam4.1018
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发表时间：
2017-05
期刊：
影响因子：
4
通讯作者：
Zhu J
中科院分区：
文献类型：
--
作者：
Zhao W;Ding G;Wen J;Tang Q;Yong H;Zhu H;Zhang S;Qiu Z;Feng Z;Zhu J
胃癌是一种多步骤、多阶段的疾病，绝大多数胃癌细胞可过表达一种或多种癌基因。Trop 2和双调蛋白（AREG）在多种上皮细胞癌中均过表达，并在增加肿瘤细胞分裂和转移中起作用。然而，很少有人知道的功能和相关性的两个癌基因共表达的GC。检测两种基因在791例胃癌组织中的表达水平，并通过免疫组化（IHC）分析两种基因表达与胃癌患者临床病理特征及总生存期的相关性。本研究还通过qRT-PCR检测了26例新鲜胃癌组织中两种基因的mRNA表达水平。结果表明，胃癌组织中Trop 2 +/AREG+共表达高于癌旁组织。Trop 2 +/AREG+蛋白共表达与肿瘤的TNM分期（χ 2 = 50.345，P < 0.001）、肿瘤大小（χ 2 = 40.349，P < 0.001）、淋巴结转移（χ 2 = 26.481，P < 0.001）和远处转移（χ 2 = 8.387，P = 0.039）相关。Trop 2+和AREG+蛋白同时表达的胃癌患者总生存率较低（HR = 3.682，95% CI = 2.038-6.654，P < 0.001）。Trop 2/AREG表达水平呈正相关（r = 0.254，P < 0.001）。mRNA表达结果与蛋白表达结果相似。Trop 2和AREG可作为胃癌预后的辅助生物标志物，联合检测Trop 2和AREG有助于判断胃癌患者的预后。
Gastric cancer (GC) is a multistep and multistage disease and the majority of GC cells could overexpressed one or more oncogenes. Trop2 and amphiregulin (AREG) are both overexpressed in various epithelial cell cancers and have the role in the increases tumor cells division and metastasis. However, little is known about the function and correlation of two oncogenes coexpressed in GC. The expression level of these two genes in 791 cases of GC tissues were tested, the correlations between two genes expression and clinical pathological characteristics and overall survival in GC patients through immunohistochemistry (IHC) were analyzed. This study also explored the mRNA expression level of two genes in 26 cases of freshly GC tissues by qRT‐PCR. The results indicated that Trop2+/AREG+ coexpression was higher in GC tissues than in adjacent tissues. Trop2+/AREG+ protein coexpression were associated with Tumor Node Metastasis (TNM) stage (χ 2 = 50.345, P < 0.001), tumor size (χ 2 = 40.349, P < 0.001), lymph node metastases (χ 2 = 26.481, P < 0.001), and distant metastases (χ 2 = 8.387, P = 0.039). GC patients with Trop2+ and AREG+ protein coexpression had poor overall survival rates (HR = 3.682, 95% CI = 2.038–6.654, P < 0.001). The expression level of Trop2/AREG were positively correlated (r 0.254 and P < 0.001). The result of the mRNA expression was similar to that of the protein expression. Overall, Trop2 and AREG could be seen as prognostic cobiomarker in GC and combined detection of Trop2 and AREG could be viewed as helpful in predicting the prognosis of the GC patients.