Used leucodepletion filters as a source of large quantities of DNA suitable for the study of genetic variations in human populations
Used leucodepletion filters as a source of large quantities of DNA suitable for the study of genetic variations in human populations
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DOI：
10.1046/j.1365-3148.2003.00423.x
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发表时间：
2003-04-01
影响因子：
1.5
通讯作者：
Briggs, DC
中科院分区：
文献类型：
--
作者：
Cook, MA;Jobson, SE;Briggs, DC
建立了一种简单的技术，用于开发适合于群体遗传学研究的具有大量DNA的大型对照组。背景和目的：缺乏合适的对照组和不足量的DNA用于重复分析同一对照组常常阻碍疾病遗传标记的调查。材料和方法：使用废物产品从常规献血，我们描述了一个简单的方法，允许调查员提取大量的DNA.Results：平均1520杯的DNA每个样本获得。结论：该技术可用于检测大量的基因组DNA，并可用于数千次检测。
A simple technique for developing large control panels with large quantities of DNA suitable for studies in population genetics was established.Background and objectives: Both a lack of suitable controls and insufficient quantities of DNA for repeated analysis of the same control group often hamper the investigation of genetic markers for disease.Materials and methods: Using a waste product from routine blood donation, we describe a simple method that allows the investigator to extract large amounts of DNA.Results: A mean of 1520 mug of DNA per sample was obtained. The DNA obtained remains suitable for polymerase chain reaction and sequencing techniques after 2 years of storage at both 4degreesC and -40degreesC.Conclusion: This technique allows the development of a large panel of controls with sufficient quantities of genomic DNA for thousands of tests.