Strain differentiation of isolates of streptococci from bovine mastitis by pulsed-field gel electrophoresis
Strain differentiation of isolates of streptococci from bovine mastitis by pulsed-field gel electrophoresis
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DOI：
10.1006/mcpr.1997.0126
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发表时间：
1997-10-01
影响因子：
3.3
通讯作者：
Browning, GF
中科院分区：
文献类型：
--
作者：
Baseggio, N;Mansell, PD;Browning, GF
脉冲场凝胶电泳（PFGE）检查作为一种工具，以区分从临床和亚临床病例的牛乳房炎分离的链球菌菌株。无乳链球菌SmaI染色体多态性分析停乳亚种dysgalactiae和S.分离株揭示了每个种属内的群内和群间菌株关系。比较S。来自同一牛群的无乳链球菌分离株在它们的SmaI限制性酶切图谱中显示出很小的变异性，表明单一菌株来自共同的感染源。然而，S.牛群之间的无乳分离株表明，每个牛群都感染了不同的菌株。S.停乳亚种dysgalactiae和S.牛群的种类更为多样，通常在一个牛群中存在两个种属的多个菌株，有些牛群含有一个以上的特定菌株代表。有趣的是，只有S。停乳亚种停乳症，一些菌株存在于一个以上的牛群。PFGE被认为是一种有用的和可重复的方法，用于区分不同菌株的三个最重要的物种的链球菌负责牛乳腺炎，并提供一种手段来识别感染的环境来源。(C)出版社：Academic Press Limited。
Pulsed-field gel electrophoresis (PFGE) was examined as a tool to differentiate strains of streptococci isolated from clinical and sub-clinical cases of bovine mastitis. Analysis of SmaI chromosomal digests of Streptococcus agalactiae, S. dysgalactiae subsp. dysgalactiae and S. uberis isolates revealed intraherd and interherd strain relationships within each species. Comparison of S. agalactiae isolates from the same herd revealed little variability in their SmaI restriction patterns indicating a single strain originating from a common source of infection. However, comparison of S. agalactiae isolates between herds showed that each herd was infected with a distinct strain. The restriction profiles from S. dysgalactiae subsp. dysgalactiae and S. uberis were more diverse and often multiple strains of both species were present within an individual herd, with some herds containing more than one representative of a particular strain. Interestingly, it was only observed with S. dysgalactiae subsp. dysgalactiae that some strains were present in more than one herd. PFGE was found to be a useful and reproducible method for the discrimination of different strains of the three most important species of streptococci responsible for bovine mastitis and to offer a means to identify environmental sources of infection. (C) 1997 Academic Press Limited.