Influence of odorant receptor repertoire on odor perception in humans and fruit flies
Influence of odorant receptor repertoire on odor perception in humans and fruit flies
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DOI：
10.1073/pnas.0605321104
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发表时间：
2007-03-27
影响因子：
11.1
通讯作者：
Vosshall, Leslie B.
中科院分区：
文献类型：
--
作者：
Keller, Andreas;Vosshall, Leslie B.
嗅觉系统被认为通过多个气味受体（OR）识别气味，这些气味受体被重叠的气味分子激活，最终在大脑中产生气味受体。我们研究了人类和果蝇之间的气味感受器的差异，具有非常不同的OR剧目的物种。我们设计了高通量的单飞行为范例，以询问给定的OR如何有助于果蝇的气味感受。野生型苍蝇表现出剂量和刺激依赖性的反应73气味测试的70，而突变苍蝇失踪一个OR表现出微妙的行为缺陷，不能预测的生理反应的OR。我们测量了人类和苍蝇的气味强度和质量的判断，发现强度感知是物种之间的保守，而质量判断是物种特异性的。这项研究填补了嗅觉感觉神经元激活和气味感受之间的差距。
The olfactory system is thought to recognize odors with multiple odorant receptors (ORs) that are activated by overlapping sets of odorous molecules, ultimately generating an odor percept in the brain. We investigated how the odor percept differs between humans and Drosophila melanogaster fruit flies, species with very different OR repertoires. We devised high-throughput single fly behavior paradigms to ask how a given OR contributes to the odor percept in Drosophila. Wild-type flies showed dose- and stimulus-dependent responses to 70 of 73 odors tested, whereas mutant flies missing one OR showed subtle behavioral deficits that could not be predicted from the physiological responses of the OR. We measured human and fly judgments of odor intensity and quality and found that intensity perception is conserved between species, whereas quality judgments are species-specific. This study bridges the gap between the activation of olfactory sensory neurons and the odor percept.