Early detection of cognitive deficits in the 3xTg-AD mouse model of Alzheimer's disease
Early detection of cognitive deficits in the 3xTg-AD mouse model of Alzheimer's disease
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DOI：
10.1016/j.bbr.2015.04.012
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发表时间：
2015-08-01
影响因子：
2.7
通讯作者：
Brown, Richard E.
中科院分区：
文献类型：
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作者：
Stover, Kurt R.;Campbell, Mackenzie A.;Brown, Richard E.
哪种行为测试对检测6.5月龄3xTg-AD的认知缺陷最敏感？阿尔茨海默病(AD)的3xTg-AD小鼠模型有三个转基因(APPswe、PS1M146V和Tau P301L)，它们会导致随着年龄的增长而发生淀粉样β斑块、神经原纤维缠结和认知障碍。为了确定哪项任务在认知缺陷的早期检测中最敏感，我们对6.5月龄的雄性和雌性3xTg-AD和B6129SF2野生型小鼠进行了测试，包括Y迷宫中的自发交替、新对象识别、巴恩斯迷宫中的空间记忆以及线索和情景恐惧条件反射。3xTg-AD小鼠在Barnes迷宫中的学习和记忆能力受损，但在Y迷宫和情景恐惧条件反射中的表现优于B6129SF2野生型小鼠。在新的物体识别任务中，两种基因都没有表现出偏爱，在线索恐惧条件反射中也没有基因差异，但女性的表现好于男性。根据我们的结果，我们得出结论，3xTg-AD小鼠在空间学习和记忆方面存在轻度认知缺陷，Barnes迷宫是检测6.5月龄小鼠这些认知缺陷的最敏感的测试。(C)2015爱思唯尔B.V.保留所有权利。
Which behavioral test is the most sensitive for detecting cognitive deficits in the 3xTg-AD at 6.5 months of age? The 3xTg-AD mouse model of Alzheimer's disease (AD) has three transgenes (APPswe, PS1M146V, and Tau P301L) which cause the development of amyloid beta plagues, neurofibrillary tangles, and cognitive deficits with age. In order to determine which task is the most sensitive in the early detection of cognitive deficits, we compared male and female 3xTg-AD and B6129SF2 wildtype mice at 6.5 months of age on a test battery including spontaneous alternation in the Y-Maze, novel object recognition, spatial memory in the Barnes maze, and cued and contextual fear conditioning. The 3xTg-AD mice had impaired learning and memory in the Barnes maze but performed better than B6129SF2 wildtype mice in the Y-Maze and in contextual fear conditioning. Neither genotype demonstrated a preference in the novel object recognition task nor was there a genotype difference in cued fear conditioning but females performed better than males. From our results we conclude that the 3xTg-AD mice have mild cognitive deficits in spatial learning and memory and that the Barnes maze was the most sensitive test for detecting these cognitive deficits in 6.5-month-old mice. (C) 2015 Elsevier B.V. All rights reserved.