Investigating the effect of chromatographic conditions on retention of organic acids in hydrophilic interaction chromatography using a design of experiment
Investigating the effect of chromatographic conditions on retention of organic acids in hydrophilic interaction chromatography using a design of experiment
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DOI：
10.1365/s10337-007-0264-0
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发表时间：
2007-08-01
期刊：
影响因子：
1.7
通讯作者：
Gaiki, S.
中科院分区：
文献类型：
--
作者：
Guo, Y.;Srinivasan, S.;Gaiki, S.
小分子有机酸在各种固定相上都有很好的保留，如酰胺相、氨基相、异酰胺相、硅胶相和磺基甜菜碱相。本文采用实验设计（DOE）方法，考察了色谱条件对有机酸在HILIC中保留行为的影响。DOE研究的结果表明，有机溶剂的含量（即，乙腈）和移动的相中的盐浓度对有机酸的保留有显著影响。移动的相中有机溶剂含量的增加导致了所有类型的固定相上的保留值的显著增加。增加盐浓度也导致保留的适度增加;然而，盐浓度的影响因固定相的类型而异。研究还发现，柱温对保留值的影响小于有机溶剂含量和盐浓度。
Small organic acids have shown significant retention on various stationary phases, such as amide, amino, aspartamide, silica and sulfobetaine phase commonly used in hydropkilic interaction chromatography (HILIC). This study investigated the effect of chromatographic conditions on the retention behavior of organic acids in HILIC using the tool of design of experiment (DOE). The results of the DOE study indicated that both the content of organic solvent (i.e., acetonitrile) and salt concentration in the mobile phase had significant effects on the retention of organic acids. Higher content of organic solvent in the mobile phase led to a significant increase in retention on all types of stationary phases. Increasing salt concentration also resulted in a moderate increase in retention; however, the effect of salt concentration varied with the type of stationary phase. The study also revealed that column temperature had less impact on retention than organic solvent content and salt concentration in HILIC.