Dynamic behavior of transcription factors on a natural promoter in living cells
Dynamic behavior of transcription factors on a natural promoter in living cells
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DOI：
10.1093/embo-reports/kvf244
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发表时间：
2002-12-01
期刊：
影响因子：
7.7
通讯作者：
Hager, GL
中科院分区：
文献类型：
--
作者：
Becker, M;Baumann, C;Hager, GL
通过使用光漂白技术，我们研究了活细胞中转录装置的三个成员与靶启动子的动态相互作用。糖皮质激素受体（GR）相互作用蛋白1（GRIP-1）表现出荧光恢复（tau（R））的半最大时间为5秒，反映了相同的快速交换观察GR。相反，大亚基（RPB 1）的RNA聚合酶II（pol II）需要13分钟的完全荧光恢复，符合其功能作为一个进行性酶。我们还观察到GR刺激转录动力学的复杂诱导特征。我们的研究结果表明，GR和GRIP-1作为激活复合物的组成部分，与启动子处于动态平衡，并且在转录激活过程中必须多次返回模板。
Through the use of photobleaching techniques, we examined the dynamic interaction of three members of the transcription apparatus with a target promoter in living cells. The glucocorticoid receptor (GR) interacting protein 1 (GRIP-1) exhibits a half maximal time for fluorescent recovery (tau(R)) of 5 s, reflecting the same rapid exchange as observed for GR. In contrast, the large subunit (RPB1) of RNA polymerase II (pol II) required 13 min for complete fluorescence recovery, consistent with its function as a processive enzyme. We also observe a complex induction profile for the kinetics of GR-stimulated transcription. Our results indicate that GR and GRIP-1 as components of the activating complex are in a dynamic equilibrium with the promoter, and must return to the template many times during the course of transcriptional activation.