DETERMINATION OF CHLORHEXIDINE IN SALIVA AND IN AQUEOUS-SOLUTIONS
DETERMINATION OF CHLORHEXIDINE IN SALIVA AND IN AQUEOUS-SOLUTIONS
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DOI：
10.1159/000261403
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发表时间：
1991-11-01
期刊：
影响因子：
4.2
通讯作者：
ARENDS, J
中科院分区：
文献类型：
--
作者：
DEVRIES, J;RUBEN, J;ARENDS, J
建立了用荧光光谱法测定离心唾液和水溶液中氯己定含量的新方法。该方法依赖于氯己定与伊红之间的络合物形成。氯己定-伊红体系的荧光值随氯己定浓度的增加而降低。在离心后的唾液中，541 nm处的荧光与氯己定含量呈线性关系，最高可达15 ppm；结果表明，该方法的重现性优于1 PPM氯己定。不同参与者离心后唾液的生物扩散和唾液收集周期引起的扩散约为3%。在整个未离心的唾液中，由于氯己定与唾液成分结合，荧光法的检测限约为8ppm。超过8 ppm的氯己定，唾液中的生物变化是这样的，用荧光方法测定氯己定只能在+/- 10 ppm的重现性下进行。讨论了紫外光谱法、放射性标记法和荧光法测定氯己定的优缺点。
A new method is presented for the determination of chlorhexidine in centrifuged saliva and in aqueous solutions by means of fluorescence spectroscopy. The method relies on complex formation between chlorhexidine and eosin. The fluorescence value of the chlorhexidine-eosin system decreases with increasing chlorhexidine concentrations. In centrifuged saliva a linear relation was found between the fluorescence at 541 nm and the chlorhexidine content up to about 15 ppm; the reproducibility of the method was found to be better than 1 ppm chlorhexidine. The biological spreading for centrifuged saliva from different participants as well as the spreading due to the period of saliva collection are about 3%. In whole uncentrifuged saliva the fluorescence method has been a detection limit of about 8 ppm chlorhexidine, due to the binding of the compound to salivary constituents. Above 8 ppm chlorhexidine, the biological variation in saliva is such that chlorhexidine determinations by means of fluorescence can be done only with a reproducibility of +/- 10 ppm. Advantages and disadvantages of the ultraviolet spectroscopy method, the radioactive labelling technique, and the fluorescence method for chlorhexidine determinations are discussed.