High levels of Tryptophan reduce cell wall or membrane stress tolerance in Saccharomyces cerevisiae
High levels of Tryptophan reduce cell wall or membrane stress tolerance in Saccharomyces cerevisiae
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DOI：
10.1093/bbb/zbab142
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发表时间：
2021-08-13
影响因子：
1.6
通讯作者：
Chaleckis, Romanas
中科院分区：
文献类型：
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作者：
Ohashi, Kazuto;Chaleckis, Romanas
色氨酸 (Trp) 是一种蛋白质芳香氨基酸；然而，高浓度的色氨酸对动物和酵母具有毒性，其机制尚不清楚。之前，我们认为芳香族转氨酶 Aro9 对于过度的色氨酸降解很重要。此外，Schroeder和Ikui表明aro9 Delta对十二烷基硫酸钠的膜应激敏感。因此，TV 积累可能会通过细胞壁完整性 (CWI) 途径的参与来降低细胞壁或膜 (CW/M) 应激耐受性，该途径检测并响应 CW/M 扰动。在这项研究中，我们发现 CWI 丝裂原激活蛋白激酶级联的酵母突变体对过量的色氨酸敏感。此外，Trp 降解缺陷突变体 aro8 Delta aro9 Delta 细胞（其中色氨酸积累得到证实）对几种 CW/M 胁迫敏感。这些结果表明，Trp 的积累不利于 CW/M 应激抵抗，并可能干扰响应应激的适当信号转导。[图表]。
Tryptophan (Trp) is a proteinogenic aromatic amino acid; however, high levels of Trp are toxic in animals and yeast with unknown mechanisms. Previously, we suggested that aromatic aminotransferase Aro9 is important for excess Trp degradation. Besides, Schroeder and Ikui showed that aro9 Delta is sensitive to membrane stress by sodium dodecyl sulfate. Therefore, TV accumulation may reduce the cell wall or membrane (CW/M) stress tolerance through participation of cell wall integrity (CWI) pathway, which detects and responds to CW/M perturbations. In this study, we found that yeast mutants of the CWI mitogen-activated protein kinase cascade were susceptible to excess Trp. Also, the Trp degradation deficient mutant aro8 Delta aro9 Delta cells, in which Trp accumulation was confirmed, were sensitive to several CW/M stresses. These results indicated that accumulation of Trp is adverse for the CW/M stress resistance and may disturb appropriate signal transduction responding to the stress.[GRAPHICS].