Genipin crosslinking of cartilage enhances resistance to biochemical degradation and mechanical wear.
Genipin crosslinking of cartilage enhances resistance to biochemical degradation and mechanical wear.
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DOI：
10.1002/jor.22939
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Wagner DR
中科院分区：
文献类型：
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作者：
McGann ME;Bonitsky CM;Jackson ML;Ovaert TC;Trippel SB;Wagner DR
胶原交联增强了关节软骨的许多有益特性，包括抗化学降解和机械磨损，但许多交联剂具有细胞毒性。本研究的目的是评估genipin的有效性，genipin是一种具有良好生物相容性和细胞毒性的交联剂，作为防止关节软骨降解和磨损的潜在治疗方法。首先，量化了吉尼平浓度和处理时间对牛关节软骨粘弹性的影响。接下来，选择两种短期（15分钟）的genipin交联处理，测量这些处理对胶原酶消化、软骨磨损和组织摩擦系数的变化。最后，评估暴露于这些吉尼平治疗后的软骨细胞活力。Genipin治疗以剂量依赖的方式增加了健康、完整软骨的硬度。15分钟的交联治疗提高了软骨对化学降解的抵抗力，特别是在关节表面，以及由于机械磨损造成的损伤。这些增强是在不牺牲组织的低摩擦系数的情况下实现的，并且在genipin剂量下保留了软骨细胞的活力。本研究的结果表明，胶原交联通过genipin可能是一个有希望的预防性治疗，以减缓软骨的降解。
Collagen crosslinking enhances many beneficial properties of articular cartilage, including resistance to chemical degradation and mechanical wear, but many crosslinking agents are cytotoxic. The purpose of this study was to evaluate the effectiveness of genipin, a crosslinking agent with favorable biocompatibility and cytotoxicity, as a potential treatment to prevent the degradation and wear of articular cartilage. First, the impact of genipin concentration and treatment duration on the viscoelastic properties of bovine articular cartilage was quantified. Next, two short-term (15 minute) genipin crosslinking treatments were chosen, and the change in collagenase digestion, cartilage wear, and the friction coefficient of the tissue with these treatments was measured. Finally, chondrocyte viability after exposure to these genipin treatments was assessed. Genipin treatment increased the stiffness of healthy, intact cartilage in a dose-dependent manner. The 15-minute crosslinking treatments improved cartilage's resistance to both chemical degradation, particularly at the articular surface, and to damage due to mechanical wear. These enhancements were achieved without sacrificing the low coefficient of friction of the tissue and at a genipin dose that preserved chondrocyte viability. The results of this study suggest that collagen crosslinking via genipin may be a promising preventative treatment to slow the degradation of cartilage.