Label-free, sensitive detection of Hg(II) with gold nanoparticles by using dynamic light scattering technique
Label-free, sensitive detection of Hg(II) with gold nanoparticles by using dynamic light scattering technique
复制标题
使用动态光散射技术，利用金纳米颗粒进行无标记、灵敏的 Hg(II) 检测
DOI：
10.1016/j.talanta.2011.12.036
复制
发表时间：
2012-01-30
期刊：
影响因子：
6.1
通讯作者：
Ling, Liansheng
中科院分区：
文献类型：
--
作者：
Xiong, Cen;Ling, Liansheng
利用动态光散射(DLS)技术，建立了金纳米颗粒(AuNPs)无标记灵敏检测Hg2+的方法。荧光共振能量转移实验和熔融温度变化实验证实，5‘-TTT、CTT、CTT、CGT、TGT、TGT、TT-3’寡核苷酸在加入Hg2+离子后，可以从非卷曲结构转变为刚性双链或发夹结构。DNA结构的变化降低了其在AuNPs表面的吸附能力，导致了AuNPs在盐溶液中的聚集，用DLS技术可以用平均流体力学直径来估算。在最佳条件下，平均粒径与Hg2+浓度在0.75~25 nM范围内呈线性关系，线性回归方程为D=46.7+2.0C(NM，R=0.9958)，检出限为0.43 nM。(C)2011爱思唯尔B.V.保留所有权利。
A label-free method for sensitive detection of Hg2+ was developed with gold nanoparticles (AuNPs) by using dynamic light scattering (DLS) technique. Oligonucleotide 5'-TTT CTT CTT CGT TGT TGT TT-3' could transform from uncoil to rigid duplex or hairpin structure upon addition of Hg2+ ions, which was confirmed by experiments of fluorescence resonance energy transfer and change of melting temperature. The change of DNA structure reduced its adsorption ability on the surface of AuNPs, and resulted in the aggregation of AuNPs in the salt solution, which could be estimated with average hydrodynamic diameter by using DLS technique. Under the optimum conditions, the average diameter increased linearly with the concentration of Hg2+ over the range from 0.75 nM to 25 nM, the linear regression equation was D = 46.7 + 2.0 C (nM, R = 0.9958), with a detection limit of 0.43 nM. (C) 2011 Elsevier B.V. All rights reserved.