Drosophila fasciclinII is required for the formation of odor memories and for normal sensitivity to alcohol
Drosophila fasciclinII is required for the formation of odor memories and for normal sensitivity to alcohol
复制标题
DOI：
10.1016/s0092-8674(01)00386-5
复制
发表时间：
2001-06-15
期刊：
影响因子：
64.5
通讯作者：
Davis, RL
中科院分区：
文献类型：
--
作者：
Cheng, YZ;Endo, K;Davis, RL
果蝇fasiclinii （fasII）突变体由于编码、稳定或检索短期记忆的缺陷，在嗅觉调节后表现不佳。在训练和立即测试之前，通过诱导正常转基因的表达来挽救性能。训练后但测试前的诱导未能挽救表现，表明Fas II在记忆检索过程中并不具有排他性作用。当初始表现正常化时，fasII突变体的气味记忆稳定性与对照动物没有区别。像其他几种缺乏气味学习的突变体一样，fasII突变体对乙醇蒸气表现出高度的敏感性。因此，行为和遗传策略的结合揭示了Fas II在编码短期气味记忆和赋予酒精敏感性的分子操作中的作用。Fasⅱ在蘑菇体神经元轴突中的优先表达进一步表明，短期气味记忆在这些神经突中形成。
Drosophila fasciclinII (fasII) mutants perform poorly after olfactory conditioning due to a defect in encoding, stabilizing, or retrieving short-term memories. Performance was rescued by inducing the expression of a normal transgene just before training and immediate testing. Induction after training but before testing failed to rescue performance, showing that Fas II does not have an exclusive role in memory retrieval processes. The stability of odor memories in fasII mutants are indistinguishable from control animals when initial performance is normalized. Like several other muta nts deficient in odor learning, fasII mutants exhibit a heightened sensitivity to ethanol vapors. A combination of behavioral and genetic strategies have therefore revealed a role for Fas II in the molecular operations of encoding short-term odor memories and conferring alcohol sensitivity. The preferential expression of Fas II in the axons of mushroom body neurons furthermore suggests that short-term odor memories are formed in these neurites.