Inhibition of sucrose loss during cold storage in Japanese pear (Pyrus pyrifolia Nakai) by 1-MCP
Inhibition of sucrose loss during cold storage in Japanese pear (Pyrus pyrifolia Nakai) by 1-MCP
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DOI：
10.1016/j.postharvbio.2007.10.015
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发表时间：
2008-06-01
影响因子：
7
通讯作者：
Tanahashi, Takuro
中科院分区：
文献类型：
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作者：
Itai, Akihiro;Tanahashi, Takuro
在储存期间保持水果的味道对许多水果种类都很重要，包括日本梨。由于糖是水果味道和品质的最重要的组成部分之一，我们研究了在日本梨（梨Nakai cvs.黄金二世和丰水）。室温贮藏（对照）导致果实迅速软化和叶绿素降解，而这些过程被冷藏延迟。此外，与单独的冷处理相比，用1-MCP（1-甲基环丙烯）的额外处理将储存期延长了一个月。因此，低温加1-MCP处理是长期贮藏的最有效方法。在所有处理中，总糖含量在储存期间保持不变。然而，冷藏导致了己糖的积累和蔗糖的减少。冷藏1个月后，蔗糖含量几乎为零，而对照果实保持不变。然而，额外的处理与1-MCP抑制蔗糖损失和积累的己糖。为了研究蔗糖损失是否与蔗糖代谢酶（蔗糖合成酶，SS）、可溶性酸性转化酶（AIV）和蔗糖磷酸合成酶（SPS）的表达变化有关，我们检测了4个基因在贮藏过程中的表达，包括SS（PpSUSI）、AIV（PpAIVI和PpAIV 2）和SPS（PpSPSI）。冷处理增加了PpAIVI的表达，特别是在储存14天后，并导致PpSPSI转录本略有下降。冷加1-MCP抑制PpAIVI的积累和PpSPSI的降低。PpAIV 2和PpSUSI表达在很大程度上不受冷处理的影响。冷藏过程中PPAIVI转录的增加和PpSPSI转录的减少可能导致己糖的积累和蔗糖的减少。(C)2007 Elsevier B. V.保留所有权利。
Maintaining fruit taste during storage is important in many fruit species, including Japanese pear. Since sugars are one of the most important components of fruit taste and quality, we examined changes in sugar metabolism during storage in Japanese pear (Pyrus pyrifolia Nakai cvs. Gold Nijisseiki and Hosui). Storage at room temperature (control) resulted in rapid fruit softening and chlorophyll degradation, whereas these processes were delayed by cold storage. Moreover, additional treatment with 1-MCP (1-methylcyclopropene) extended storage periods for another month compared with cold treatment alone. Thus, cold temperature plus 1-MCP treatment is the most effective method for long storage. Total sugar content was maintained during storage in all treatments. However, cold storage led to an accumulation of hexoses and a decrease of sucrose. After I month of cold storage, sucrose content was almost zero, but was maintained in control fruit. However, additional treatment with 1-MCP inhibited sucrose loss and accumulation of hexoses. To investigate whether sucrose loss was related to changes in the expression of sucrose metabolizing enzymes (sucrose synthase, SS; soluble acid invertase, AIV and sucrose phosphate synthase, SPS), we examined the expression of four genes, including SS (PpSUSI), AIV (PpAIVI and PpAIV2) and SPS (PpSPSI) during storage. Cold treatment increased expression of PpAIVI, especially after 14 days of storage and resulted in a slight decrease in PpSPSI transcripts. Cold plus 1-MCP inhibited accumulation of PpAIVI and a decrease in PpSPSI. PpAIV2 and PpSUSI expression was largely unaffected by cold treatment. The increase in PPAIVI transcript through cold storage with the concomitant decrease of PpSPSI transcript may lead to an accumulation of hexoses and a decrease of sucrose. (C) 2007 Elsevier B.V. All rights reserved.