Creatine supplementation augments the increase in satellite cell and myonuclei number in human skeletal muscle induced by strength training
Creatine supplementation augments the increase in satellite cell and myonuclei number in human skeletal muscle induced by strength training
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DOI：
10.1113/jphysiol.2006.107359
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发表时间：
2006-06-01
影响因子：
5.5
通讯作者：
Kjaer, Michael
中科院分区：
文献类型：
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作者：
Olsen, Steen;Aagaard, Per;Kjaer, Michael
本研究研究了在16周的大阻力训练中补充肌酸和蛋白质对人类骨骼肌卫星细胞频率和肌核数量的影响。在双盲设计中，32名健康男性受试者（19-26岁）被分配进行力量训练（STR），同时接受定时摄入肌酸（STR- cre） （n = 9）、蛋白质（STR- pro） （n = 8）或安慰剂（STR-CON） （n = 8），或作为非训练对照组（CON） （n = 7）。每日给予补充（STR-CRE: 6-24 g一水肌酸，STR-PRO: 20 g蛋白质，STR-CON：安慰剂）。此外，在所有训练过程中，定时摄入蛋白质/安慰剂。在阻力训练的第0、4、8周（第8周，非CON）和第16周（每周3天）进行肌肉活检。卫星细胞免疫组化鉴定。组织化学分析测定肌肉平均纤维（MFA）面积。所有训练方案均可增加卫星细胞的比例，但在第4周（与STR-CON相比）和第8周（与STR-PRO和STR-CON相比）补充肌酸可显著提高卫星细胞的比例（P < 0.01-0.05）。第16周时，STR-CRE组卫星细胞数不再升高，而STR-PRO和STR-CON组卫星细胞数仍升高。此外，在第4、8和16周，补充肌酸增加了每根纤维的肌核数，MFA增加了14-17% （P < 0.01）。相比之下，STR-PRO仅在训练后期（16周，+8%）和STR-CON仅在训练早期（第4周，+14%）显示MFA增加（P < 0.05）。在STR-CRE中，MFA和myonuclei从基线到第16周的百分比分别呈正相关（r = 0.67, P < 0.05）。对照组（CON）未见变化。总之，本研究首次证明，肌酸补充与力量训练相结合，可以放大训练诱导的人体骨骼肌纤维中卫星细胞数量和肌核浓度的增加，从而增强肌纤维对力量训练的反应。
The present study investigated the influence of creatine and protein supplementation on satellite cell frequency and number of myonuclei in human skeletal muscle during 16 weeks of heavy-resistance training. In a double-blinded design 32 healthy, male subjects (19-26 years) were assigned to strength training (STR) while receiving a timed intake of creatine (STR-CRE) (n = 9), protein (STR-PRO) (n = 8) or placebo (STR-CON) (n = 8), or serving as a non-training control group (CON) (n = 7). Supplementation was given daily (STR-CRE: 6-24 g creatine monohydrate, STR-PRO: 20 g protein, STR-CON: placebo). Furthermore, timed protein/placebo intake were administered at all training sessions. Muscle biopsies were obtained at week 0, 4, 8 (week 8 not CON) and 16 of resistance training (3 days per week). Satellite cells were identified by immunohistochemistry. Muscle mean fibre (MFA) area was determined after histochemical analysis. All training regimes were found to increase the proportion of satellite cells, but significantly greater enhancements were observed with creatine supplementation at week 4 (compared to STR-CON) and at week 8 (compared to STR-PRO and STR-CON) (P < 0.01-0.05). At week 16, satellite cell number was no longer elevated in STR-CRE, while it remained elevated in STR-PRO and STR-CON. Furthermore, creatine supplementation resulted in an increased number of myonuclei per fibre and increases of 14-17% in MFA at week 4, 8 and 16 (P < 0.01). In contrast, STR-PRO showed increase in MFA only in the later (16 week, +8%) and STR-CON only in the early (week 4, +14%) phases of training, respectively (P < 0.05). In STR-CRE a positive relationship was found between the percentage increases in MFA and myonuclei from baseline to week 16, respectively (r = 0.67, P < 0.05). No changes were observed in the control group (CON). In conclusion, the present study demonstrates for the first time that creatine supplementation in combination with strength training amplifies the training-induced increase in satellite cell number and myonuclei concentration in human skeletal muscle fibres, thereby allowing an enhanced muscle fibre growth in response to strength training.