GLUCOSE-TRANSPORTER LOCALIZATION IN RAT SKELETAL-MUSCLE - AUTORADIOGRAPHIC STUDY USING ATB-[2-H-3]BMPA PHOTOLABEL
GLUCOSE-TRANSPORTER LOCALIZATION IN RAT SKELETAL-MUSCLE - AUTORADIOGRAPHIC STUDY USING ATB-[2-H-3]BMPA PHOTOLABEL
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DOI：
10.1016/0014-5793(94)80416-8
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发表时间：
1994-02-21
期刊：
影响因子：
3.5
通讯作者：
WILSON, CM
中科院分区：
文献类型：
--
作者：
DUDEK, RW;DOHM, GL;WILSON, CM
用ATB-[2-H-3]BMPA试剂光标记完整肌肉的表面葡萄糖转运体，并用放射自显影进行定位。我们发现ATB-[2-H-3]BMPA在肌纤维周围的肌膜上有稀疏的葡萄糖转运蛋白标记。大多数标记位于肌纤维的内部，位于与AI连接分布相匹配的离散位置，推测位于T管的外表面。在胰岛素刺激下，T小管上的光标记物数量增加，并被细胞松弛素B阻断。这些结果支持了葡萄糖在基础和胰岛素刺激条件下可能主要通过T小管转运的概念。
Surface glucose transporters of intact muscles were photolabeled with the membrane impermeant ATB-[2-H-3]BMPA reagent and localized by autoradiography. We found sparse labeling of the glucose transporters by ATB-[2-H-3]BMPA on the sarcolemmal membrane around the muscle fiber. The majority of label was on the interior of the muscle fiber, at a discrete site which matched the distribution of AI junctions and which was presumed to be on the exterior surface of T-tubules. The amount of photolabel on the T-tubule was increased in response to insulin and was blocked by cytochalasin B. These results support the concept that glucose transport may occur predominately across the T-tubule membrane under basal and insulin-stimulated conditions.