The effects of a co-application of menthol and capsaicin on nociceptive behaviors of the rat on the operant orofacial pain assessment device.
The effects of a co-application of menthol and capsaicin on nociceptive behaviors of the rat on the operant orofacial pain assessment device.
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DOI：
10.1371/journal.pone.0089137
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Neubert JK
中科院分区：
文献类型：
--
作者：
Anderson EM;Jenkins AC;Caudle RM;Neubert JK
瞬时受体电位（TRP）阳离子通道参与热痛和冷痛的感知，并且是人类疼痛缓解的靶点。我们假设TRPV 1和TRPM 8/TRPA 1的激动剂，辣椒素和薄荷醇，将改变大鼠的伤害性行为，但它们对温度检测的相反作用将相互减弱，如果组合。在口面疼痛评估装置（OPAD，Stoelting Co.）在三个温度下在17分钟的行为会话（33°C、21°C、45°C）。舔/脸比率（L/F：奖励舔事件除以刺激接触的数量。每次有舔事件时，都进行接触。）是对OPAD的伤害感受的量度，并且这在45°C和21°C下同样降低，表明它们对大鼠都是伤害感受的和/或厌恶的。然而，大鼠在33°C和21°C下消耗（舔）等量，但在45°C下消耗较少，这表明在口面疼痛模型中，在这些温度下热比冷更具伤害性。当薄荷醇和辣椒素单独应用时，它们都诱导伤害性行为，如较低的L/F比和舔。然而，当一起施用时，21°C下的舔数等于33°C下的舔数，并且两者均显著高于45°C下的舔数。这表明当TRPM 8/TRPA 1和TRPV 1被共激活时，低温的伤害感受性较低。这些结果表明，TRP通道的共激活可以减少某些伤害性行为。这些数据表明，TRP通道调制可以选择性地影响伤害感受的动机方面，并且疼痛的某些方面可以分离，因此在临床中选择性地靶向。
Transient receptor potential (TRP) cation channels are involved in the perception of hot and cold pain and are targets for pain relief in humans. We hypothesized that agonists of TRPV1 and TRPM8/TRPA1, capsaicin and menthol, would alter nociceptive behaviors in the rat, but their opposite effects on temperature detection would attenuate one another if combined. Rats were tested on the Orofacial Pain Assessment Device (OPAD, Stoelting Co.) at three temperatures within a 17 min behavioral session (33°C, 21°C, 45°C). The lick/face ratio (L/F: reward licking events divided by the number of stimulus contacts. Each time there is a licking event a contact is being made.) is a measure of nociception on the OPAD and this was equally reduced at 45°C and 21°C suggesting they are both nociceptive and/or aversive to rats. However, rats consumed (licks) equal amounts at 33°C and 21°C but less at 45°C suggesting that heat is more nociceptive than cold at these temperatures in the orofacial pain model. When menthol and capsaicin were applied alone they both induced nociceptive behaviors like lower L/F ratios and licks. When applied together though, the licks at 21°C were equal to those at 33°C and both were significantly higher than at 45°C. This suggests that the cool temperature is less nociceptive when TRPM8/TRPA1 and TRPV1 are co-activated. These results suggest that co-activation of TRP channels can reduce certain nociceptive behaviors. These data demonstrate that the motivational aspects of nociception can be influenced selectively by TRP channel modulation and that certain aspects of pain can be dissociated and therefore targeted selectively in the clinic.
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影响因子：
3.3
作者：
Anderson, E. M.;Del Valle-Pinero, A. Y.;Suckow, S. K.;Nolan, T. A.;Neubert, J. K.;Caudle, R. M.
通讯作者：
Caudle, R. M.
影响因子：
7.4
作者：
Liu B;Fan L;Balakrishna S;Sui A;Morris JB;Jordt SE
通讯作者：
Jordt SE
TRPM8离子通道的药理阻滞改变了小鼠的冷疼痛反应。
影响因子：
3.7
作者：
Knowlton WM;Daniels RL;Palkar R;McCoy DD;McKemy DD
通讯作者：
McKemy DD
影响因子：
3.3
作者：
Gentry C;Stoakley N;Andersson DA;Bevan S
通讯作者：
Bevan S
影响因子：
3.5
作者：
Albin, Kelly C.;Carstens, Mirela Iodi;Carstens, E.
通讯作者：
Carstens, E.