BACTERIOPHAGE MU INTEGRATION - MECHANISM OF MU-INDUCED MUTATIONS
BACTERIOPHAGE MU INTEGRATION - MECHANISM OF MU-INDUCED MUTATIONS
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DOI：
10.1016/0022-2836(71)90137-9
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发表时间：
1971-01-01
影响因子：
5.6
通讯作者：
ABELSON, J
中科院分区：
文献类型：
--
作者：
BORAM, W;ABELSON, J
噬菌体Mu感染大肠埃希氏菌可导致多种基因突变频率增加。Taylor(1963)提出这些突变是由Mu原噬菌体整合到基因组中引起的。本文报道的实验表明，Mu原噬菌体位于Mu诱导突变的位置或附近，这与Taylor的建议一致。Mu诱导的λ原噬菌体结合位点和高尔基操纵子之间的chl-D突变阻止了λ转导基因。从该菌株的裂解物中分离λ转导颗粒是可能的，该裂解物携带部分Mu原噬菌体。这些颗粒被包装在λ衣壳中。对Mu诱变的进一步研究进行了讨论。
Infection ofEscherichia coliwith bacteriophage Mu leads to an increase in the mutation frequency in a wide variety of genes. Taylor (1963) has proposed that these mutations are caused by the integration of the Mu prophage into the gene.The experiments reported in this paper show that the Mu prophage is genetically located at or near the site of the Mu-induced mutation in accord with Taylor's proposal. A Mu-inducedchl-Dmutation between the λ prophage attachment site and thegaloperon prevents λ from transducinggalgenes. It is possible to isolate λ-transducing particles from lysates of this strain which carry portions of the Mu prophage. These particles are packaged in λ capsids. Further aspects of Mu mutagenesis are discussed.