Discordance of DNA methylation variance between two accessible human tissues.
Discordance of DNA methylation variance between two accessible human tissues.
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DOI：
10.1038/srep08257
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发表时间：
2015-02-09
影响因子：
4.6
通讯作者：
Kobor MS
中科院分区：
文献类型：
--
作者：
Jiang R;Jones MJ;Chen E;Neumann SM;Fraser HB;Miller GE;Kobor MS
群体表观遗传学研究一直在寻求确定特定暴露、人口统计学因素或可接近组织中疾病之间的DNA甲基化差异，但对这些组织之间个体间变异性的差异知之甚少。这项研究提出了一个匹配的外周血单核细胞（PMBCs）和颊上皮细胞（BECs），人口研究中最容易的两个组织之间的DNA甲基化差异的分析，在998启动子定位的CpG位点。具体来说，我们比较了探针方式的DNA甲基化方差，以及这种方差与两种组织中的人口统计学因素的关系。PBMCs的DNA甲基化水平总体上高于BECs，两种组织在低CpG密度的基因组区域差异最大。此外，尽管两种组织均显示出明显的探针变异性，但组织之间的特异性区域和变异幅度差异很大。最后，通过探索性关联分析，我们发现BEC和PBMC与人口统计学变量之间存在差异性关联。这里提出的工作提供了深入了解个体之间和跨组织的DNA甲基化的变异性，并有助于指导决定颊上皮或外周单核细胞的适用性，为人类群体中的表观遗传学研究探索的生物学问题。
Population epigenetic studies have been seeking to identify differences in DNA methylation between specific exposures, demographic factors, or diseases in accessible tissues, but relatively little is known about how inter-individual variability differs between these tissues. This study presents an analysis of DNA methylation differences between matched peripheral blood mononuclear cells (PMBCs) and buccal epithelial cells (BECs), the two most accessible tissues for population studies, in 998 promoter-located CpG sites. Specifically we compared probe-wise DNA methylation variance, and how this variance related to demographic factors across the two tissues. PBMCs had overall higher DNA methylation than BECs, and the two tissues tended to differ most at genomic regions of low CpG density. Furthermore, although both tissues showed appreciable probe-wise variability, the specific regions and magnitude of variability differed strongly between tissues. Lastly, through exploratory association analysis, we found indication of differential association of BEC and PBMC with demographic variables. The work presented here offers insight into variability of DNA methylation between individuals and across tissues and helps guide decisions on the suitability of buccal epithelial or peripheral mononuclear cells for the biological questions explored by epigenetic studies in human populations.
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全基因组甲基化谱揭示了人类衰老速度的定量观点。
影响因子：
16
作者：
Hannum, Gregory;Guinney, Justin;Zhao, Ling;Zhang, Li;Hughes, Guy;Sadda, SriniVas;Klotzle, Brandy;Bibikova, Marina;Fan, Jian-Bing;Gao, Yuan;Deconde, Rob;Chen, Menzies;Rajapakse, Indika;Friend, Stephen;Ideker, Trey;Zhang, Kang
通讯作者：
Zhang, Kang
影响因子：
7.7
作者：
Gutierrez-Arcelus M;Lappalainen T;Montgomery SB;Buil A;Ongen H;Yurovsky A;Bryois J;Giger T;Romano L;Planchon A;Falconnet E;Bielser D;Gagnebin M;Padioleau I;Borel C;Letourneau A;Makrythanasis P;Guipponi M;Gehrig C;Antonarakis SE;Dermitzakis ET
通讯作者：
Dermitzakis ET
影响因子：
168.9
作者：
Dick, Katherine J.;Nelson, Christopher P.;Samani, Nilesh J.
通讯作者：
Samani, Nilesh J.
DOI：
10.1073/pnas.1121249109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Lam, Lucia L.;Emberly, Eldon;Kobor, Michael S.
通讯作者：
Kobor, Michael S.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y