Mineralogical features associated with cytotoxic and proliferative effects of fibrous talc and asbestos on rodent tracheal epithelial and pleural mesothelial cells.
Mineralogical features associated with cytotoxic and proliferative effects of fibrous talc and asbestos on rodent tracheal epithelial and pleural mesothelial cells.
复制标题
DOI：
10.1006/taap.1997.8276
复制
发表时间：
1997-11
影响因子：
3.8
通讯作者：
A. Wylie;H. Skinner;J. Marsh;H. Snyder;C. Garzione;Damian G. Hodkinson;Roberta Winters;B. Mossman
中科院分区：
文献类型：
--
作者：
A. Wylie;H. Skinner;J. Marsh;H. Snyder;C. Garzione;Damian G. Hodkinson;Roberta Winters;B. Mossman
吸入石棉纤维会导致细胞损伤，并增加体内肺和胸膜各种细胞类型的细胞增殖。通过使用集落形成效率（CFE）测定，将含有不同比例纤维滑石的三种矿物样品的细胞毒性和增殖潜力与青石棉和温石棉石棉的NIEHS样品在引起气管支气管癌的细胞类型中进行比较，即，仓鼠气管上皮（HTE）细胞和间皮瘤，即，大鼠胸膜间皮细胞（RPM）。矿物组成、表面积和粒度分布以及所有矿物样品中纤维的比例的表征允许通过各种剂量参数检查数据，包括等重量浓度、长度>5微米的纤维数量和等效表面积。暴露于石棉样品导致HTE细胞的菌落数量增加，这是增殖潜力的指示，但纤维滑石粉没有。RPM没有表现出增加的CFE在石棉或滑石样品。在两种细胞类型中均观察到菌落数量减少（细胞毒性的指示），并且与滑石样本相比，在较低重量浓度的石棉下更显着。然而，当剂量被测量为大于5微米或表面积的纤维数量时，所有纤维矿物质样品产生了相当的剂量-反应效应。HTE细胞对石棉的独特增殖反应不能用纤维尺寸或表面积的差异来解释，这表明矿物成分而不是纤维尺寸的重要作用。
Inhalation of asbestos fibers causes cell damage and increases in cell proliferation in various cell types of the lung and pleura in vivo. By using a colony-forming efficiency (CFE) assay, the cytotoxicity and proliferative potential of three mineral samples containing various proportions of fibrous talc were compared to NIEHS samples of crocidolite and chrysotile asbestos in cell types giving rise to tracheobronchial carcinomas, i.e., hamster tracheal epithelial (HTE) cells, and mesotheliomas, i.e., rat pleural mesothelial (RPM) cells. Characterization of mineralogical composition, surface area, and size distributions as well as proportions of fibers in all mineral samples allowed examination of data by various dose parameters including equal weight concentrations, numbers of fibers >5 micron in length, and equivalent surface areas. Exposure to samples of asbestos caused increased numbers of colonies of HTE cells, an indication of proliferative potential, but fibrous talc did not. RPMs did not exhibit increased CFE in response to either asbestos or talc samples. Decreased numbers of colonies, an indication of cytotoxicity, were observed in both cell types and were more striking at lower weight concentrations of asbestos in comparison to talc samples. However, all samples of fibrous minerals produced comparable dose-response effects when dose was measured as numbers of fibers greater than 5 micron or surface area. The unique proliferative response of HTE cells to asbestos could not be explained by differences in fiber dimensions or surface areas, indicating an important role of mineralogical composition rather than size of fibers.